吖啶红指示催化动力学光度法测定痕量亚硝酸根

Catalytic Kinetic Spectrophotometric Method for Determination of Trace Amounts of Nitrite with Acridine Red Designating

在稀磷酸介质中 ,NO-2 能在室温下催化KBrO3 氧化吖啶红 ,产生褪色作用。据此指示反应 ,建立了催化动力学吸光光度法测定痕量亚吸酸根的新方法。选择 2 0min作为测定时间 ,可对浓度范围在 0～ 3μg/L的NO-2 进行测定 ,其检测限可达到 1 .4× 1 0 -2 μg/L ,并有良好的选择性 ,方法用于环境水样和蔬菜中痕量NO-2 的测定 。

A catalytic kinetic spectrophotometric method for determination of trace amounts of nitrite in dilute phosphoric acid medium was proposed.The method is based on the nitrite catalyzed oxidation of acridine red by potassium bromate in room temperature with the results of decoloration.By using 20min as detection time,the range of determination was 0～3μg/L and the detection limit is 1.4×10 -2 μg/L for nitrite.The method has good selectivity and has been applied to the determination of trace amounts of nitrite in environmental water and vegetable samples with satisfactory results.