内皮间充质转化与急性病毒性心肌炎早期心肌纤维化相关性分析

Correlation analysis of endothelial-mesenchymal transition with acute viral myocarditis and early myocardial fibrosis

目的 探讨心脏内皮质间充质转化（endothelial to mesenchymal transition,EndMT）与急性病毒性心肌炎纤维化的关系.方法 选取实验室Balb/c小白鼠30只,分为心肌炎组、对照组及重组人骨形成蛋白7 （recombinant human bone morphogenetic protein-7,rh-BMP-7）干预组,每组各10只.用药后7天取小鼠心脏标本,计算心脏胶原容积积分,检测小鼠心脏器官中转化生长因子β1（transforming growth factor-β1,TGF-β1）、成纤维细胞特异性蛋白1（fibroblast specific proteins-1,FSP-1）、内皮细胞标志物、Ⅰ型胶原蛋白（collagen type l,Colαl）和基因以及α-平滑肌肌动蛋白（α-smooth muscle actin,α-SMA）的表达情况.结果 与对照组相比,心肌炎组小鼠坏死心肌呈现明显纤维化,同时出现了内皮细胞表型丢失和EndMT现象.与心肌炎组小鼠相比,干预组EndMT现象和心肌纤维化较轻.结论 TGF-β1为心肌纤维化的主要介导因子,引导EndMT.抵制TGF-β1可抵制心肌纤维化过程.

Objective To investigate the relationship between endothelial mesenchymal transition and fibrosis of acute viral myocarditis. Methods 30 laboratory mice were chosen. They were divided into three groups including the myocarditis group, the control group, and the recombinant human bone morphoge- netie protein-7 （group）. The heart specimens of the mice were taken after interference for 7 days. The inte- gral of cardiac collagen volume was calculated. The expression in mice heart about transforming growth factor-β1 ,fibroblast specific proteins-1, collagen type 1 and a-smooth muscle actin were inspected. Results Compare with the comparison team, the necrosis of myocardium of the mice in myoearditis group has an obvi- ous tendency of fibrosis. Compare with the myocarditis team, the recombinant human bone morphogenetic protein-7 group has a weaker tendency of EndMT and fibrosis. Conclusions TGF-β1 belongs to a main me- diating factor with a tendency of heart fibrosis. It can guide EndMT and resist TGF-β1 and heart fibrosis.