Rho相关卷曲螺旋形成蛋白激酶1促进肝癌细胞的转移

Ectopic overexpression of ROCK1 promotes cellular invasion of hepatocellular carcinoma

目的 :探讨Rho相关卷曲螺旋形成蛋白激酶1(ROCK1)在肝癌中的表达及在肝癌侵袭中的作用。方法 :荧光定量PCR(real-time PCR)检测肝癌及癌旁组织中ROCK1的mRNA表达水平,同时检测肝癌细胞系及正常人肝细胞中ROCK1mRNA表达水平,免疫组织化学染色(IHC)法检测肝癌及癌旁组织中ROCK1蛋白的表达量,并通过在肝癌细胞株中过表达及干扰ROCK1分析ROCK1蛋白对肝癌细胞转移的作用。结果:60对样本中有42对ROCK1的mRNA表达水平在肝癌组织中高于癌旁组织,免疫组织化学染色提示肝癌组织中ROCK1蛋白表达量高于癌旁组织。在体外肝癌细胞的功能实验中,过表达ROCK1能促进HepG2细胞的侵袭和转移,而干扰ROCK1的表达能减少SMMC-7721细胞的侵袭和转移。结论:ROCK1在肝癌组织中高表达,过表达ROCK1能促进肿瘤的侵袭及转移,ROCK1可能成为临床治疗中抑制肿瘤侵袭和转移的靶点。

Objective：To detect the expression and explored the role of ROCK1 in hepatocellular carcinoma （HCC）. Methods：The expression of ROCK1 mRNA in HCC samples and cell lines was detected by real-time PCR,while the expression of ROCK1 protein in HCC tissues and adjacent non-cancer tissues was assessed by immunohistochemistry. In addition,invasion of HCC cells was observed after overexpressing or silencing ROCK1. Results：In the total of 60 paired HCC specimens, compared with the adjacent non- cancer tissues,the expression of ROCK1 mRNA was up-regulated in 42 cases （P 〈 0.05）,and the expression of ROCK1 protein in HCC tissues was higher than that in adjacent non-cancer tissues. Overexpression of ROCK1 enhanced HCC-derived HepG2 cellular invasion in vitro. In contrast,ROCK1 knockdown via RNAi markedly suppressed these phenotypes in SMMC-7721. Conclusion：This study indicates ROCK1 is frequently overexpressed in hepatocellular carcinoma,and contributes to tumor cell invasion. ROCK1 may be a novel therapeutic target for prevention and treatment of invasion in HCC.