β葡萄糖醛酸苷酶与原发性胆总管结石的关系

The expression of glucuronidase in bile of patients with primary choledocholithiasis

目的β葡萄糖醛酸苷酶(β Glucuronidase,βG)与原发性胆总管结石的关系。方法对原发性胆总管结石患者(实验组)91例和(对照组)84例行内镜下逆行胰胆管造影术(endoscopic retrogradecholangiopancreatography,ERCP),采用酶学比色法检测胆汁内、外源性βG含量。结果 (1)原发性胆总管结石与对照组患者外源性βG比较无明显差异,然而内源性9G明显升高,具有统计学意义(P<0.01);(2)不同类型原发性胆总管结石患者中,胆红素结石及混合性结石患者胆汁中内源性9G表达明显高于胆固醇结石组,具有统计学意义(P<0.01),而外源性βG表达三组间无显著差异(P>0.05)。结论内源性βG的表达上调可能在原发性胆总管结石的发生、发展中起着重要作用。

Objective Aim：To detect the expression of[ 3 glucuronidase（13G） in bile of patients with primary choledocholithiasis and investigate the relationship betweenl3G and primary choledocholithiasis. Methods ：Ninety-one patients with primary choledocholithiasis （experiment group） and 84 control subjects （control group） were included in the study. All the patients underwent endoscopic retrograde cholangiopancreatography（ERCP）. The activity of[3G were measured by enzymological method. Results （ 1 ） The activity of endogenous [ 3G in the bile from the patients with primary choledocholithiasis was significandy higher than that of the control group （P 〈 0. 01 ） , while the activity of exogenous βG from the patients with primary choledocholithiasis showed no statistical difference with control group. （2） The activity of endogenous β in the bile from the patients with bile pigment calculi and mixed calculus was significantly higher than those with cholesterol stones （P 〈 0. 01 ） , but there was no statistical difference upon exogenous βG activity among the patients with bilirubin calculus, mixed calculus and cholesterol stones. Conclusion Endogenousl3G may play a role in the formation of primary choledocholithiasis.