烟草烟雾提取物影响主动脉平滑肌细胞GATA-2的表达

Cigarette smoke extract affects the GATA-2 expression of vascular smooth muscle cells

背景:吸烟是动脉粥样硬化形成的主要危险因素之一。目的:观察烟草烟雾提取物对大鼠血管平滑肌细胞中GATA-2浓度的影响及早期生长反应因子1在其中的作用。方法:体外培养大鼠血管平滑肌细胞,用不同浓度(0,5%,10%,20%)烟草烟雾提取物刺激,采用RT-PCR的方法检测烟草烟雾提取物对GATA-2的影响。用筛选出的最适合的烟草烟雾提取物浓度处理大鼠血管平滑肌细胞不同时间(0,4,8,12,24 h)后,检测GATA-2 mRNA的变化,以及加入生长反应因子1抑制剂后GATA-2 mRNA的变化。结果与结论:与0浓度烟草烟雾提取物相比,5%低浓度烟草烟雾提取物刺激后,GATA-2 mRNA表达较10%中浓度和20%高浓度增加的更显著。不加烟草烟雾提取物组即0 h组血管平滑肌细胞中有少量GATA-2的mRNA,5%烟草烟雾提取物刺激后于4 h内开始增加,8 h达高峰。加入生长反应因子1抑制剂后5%烟草烟雾提取物诱导的血管平滑肌细胞GATA-2 mRNA表达明显降低。提示烟草烟雾提取物可通过生长反应因子1促进GATA-2增加,抑制生长反应因子1后,GATA-2的表达减少。

BACKGROUND： Smoking is one of the major risk factors for the formation of atherosclerosis. OBJECTIVE： To investigate the effect of cigarette smoke extract on the concentration of GATA-2 in the vascular smooth muscle cells and in which the role of the early growth response factor-l. METHODS： Vascular smooth muscle cells were cultured in vitro. The vascular smooth muscle cells were treated with various concentrations of cigarette smoke extract （0, 5%, 10%, 20%）, then the reverse transcription PCR was applied to detect the mRNA expression of GATA-2. The vascular smooth muscle cells were treated with cigarette smoke extracts in the optimal concentration for 0, 4, 8, 12 and 24 hours, and then the expression of GATA-2 mRNA was observed, as well as the changes of expression of GATA-2 mRNA after added with growth response factor-1. RESULTS AND CONCLUSION： Compared to the 0 concentration group, the expression of GATA-2 mRNA after treated with low concentration （5%） of cigarette smoke extract was increased more significantly than moderate concentration （10%） and high concentration （20%）. The vascular smooth muscle cells in 0 hour group expressed GATA-2 mRNA at low level. The GATA-2 mRNA began to increase within 4 hours and reached peak at the 8 hours after stimulated with cigarette smoke extract of 5% concentration. After added with growth response factor-1 inhibitors, the expression of GATA-2 mRNA in 5% cigarette smoke extract induced vascular smooth muscle cells was decreased. Cigarette smoke extract can promote the increasing of GATA-2 by growth response factor-I, while the GATA-2 expression is reduced after the inhibition of growth response factor-l.