椎间盘突出患者椎间盘Fas基因的表达

Fas gene expression of intervertebral disc in the patients with intervertebral disc herniation

背景:临床中发现腰椎间盘突出症常在一个家族中多人甚至全部发病,而且发病的部位、原因、症状基本一致,这表明基因在该种病症中扮演着重要的角色。目的:分析家族性腰椎椎间盘突出症患者椎间盘内Fas凋亡基因表达的特点。方法:用半定量RT-PCR方法检测15例家族性腰椎椎间盘突出患者、21例普通椎间盘突出患者、5例新鲜尸体椎间盘的软骨终板和髓核组织中Fas基因的表达情况。结果与结论:家族性椎间盘突出与普通椎间盘突出患者终板内Fas基因的表达均高于新鲜尸体椎间盘终板内Fas基因的表达,差异有显著性意义(P<0.05),家族性椎间盘突出与普通椎间盘突出患者终板内Fas基因的表达差异无显著性意义(P>0.05)。家族性椎间盘突出患者髓核内Fas基因表达与普通椎间盘突出患者、新鲜尸体髓核内Fas基因表达相比较,差异无显著性意义(P>0.05)。结果可见家族性椎间盘终板中Fas基因的表达增加可能是继发性的,可以通过阻止终板退变来达到阻止椎间盘退变的目的。

BACKGROUND：The clinical research have found that the interbervebral disc herniation often occurs in several members or even al the members of a family, and the location, reason and symptom are basical y the same, indicating that genes play an important role in this kind of disease. OBJECTIVE：To analyze the apoptosis Fas gene expression characteristics of lumbar disc in the familial patients with intervertebral disc herniation. METHODS：Semi-quantitative reverse transcription-PCR was used to test Fas gene expression of vertebral pulp and cartilage endplate in the intervertebral disc among 15 familial patients, 21 ordinary patients and five fresh cadavers. RESULTS AND CONCLUSION：Fas gene expression level of endplate of familial and ordinary patients with intervertebral disc herniation was higher than that of fresh cadavers, and there was no significant difference （P〈0.05）;there was no significant difference in Fas gene expression in endplates between familial patients and ordinary patients with intervertebral disc herniation （P〉0.05）. Compared with the vertebral pulps of ordinary patients with intervertebral disc herniation and fresh cadavers, there was no significant difference in the Fas expression of vertebral pulps of familial patients with intervertebral disc herniation （P〉0.05）. The increasing Fas gene expression may be secondary in the endplates of familial patients with intervertebral disc herniation, which can prevent intervertebral disc degeneration through preventing the endplate degeneration.