维生素D3对自发性高血压大鼠靶器官炎症的影响

Effects of vitamin D3 on inflammation in hypertensive target organs in spontaneous hypertensive rats

目的探索维生素D3与高血压和炎症的关系。方法自发性高血压大鼠20只,随机分为对照组和实验组,各10只。实验组大鼠腹腔注射维生素D3制剂3μg/kg(溶于20%丙二醇0.5 mL中),每周2次;对照组仅腹腔注射丙二醇0.5 mL,两组均干预12周。实验过程中监测大鼠血压变化。干预前后,酶联免疫法检测血清25(OH)D3、钙、白细胞介素-6(IL-6)、基质金属蛋白酶-9(MMP-9)的浓度;计算肾脏-体重比和心脏-体重比;HE染色观察两组大鼠肾脏、心脏、主动脉、小动脉组织病理改变。结果实验组和对照组在干预前血压无差异显著性(P>0.05);干预后,实验组和对照组大鼠平均收缩压分别为(157±9)mmHg和(173±8)mmHg(P<0.05)。实验组的血清25(OH)D3、血钙水平比对照组高(P<0.05),IL-6、MMP-9水平实验组比对照组低(P<0.05)。实验组的心脏-体重比小于对照组(P<0.05)。实验组的肾脏、心脏和小动脉高血压、炎性损害明显轻于对照组。结论规律的维生素D3用药能够抑制炎症因子IL-6、MMP-9的产生,抑制机体炎症反应,调节控制血压。

Objective To explore the relationship between vitamin D3 and inflammation in hypertension. Methods Twenty 8-week-old male spontaneous hypertensive rats were randomly divided into two groups. The rats in the experimental group received vitamin D3 3 μg/kg treatment twice a week for three months, and the rats in control group were injected with propylene glycol. Blood pressure of rats were measured once a week during the experiment period. The concentrations of serum 25 （OH）D3 , calcium, IL-6, and MMP-9 were detected by enzyme-linked imrnunosorbent before and after the experiment. The kidney/body weight ratio and heart/body weight ratio were calculated. Biopsy of the kidney, heart and artery were done for pathological examination using HE staining. Result Blood pressure between the experimental and control groups before the intervention was not significantly different （P 〉 0.05 ）. Three months later, the mean systolic blood pressure in the experimental group was （ 157 ± 9） mmHg, significantly lower than （ 173 ± 8 ） mmHg in the control group （ P 〈 0. 05）. 25 （OH） D3 and Ca concentration were higher in the experimental group than those in the control group （ P 〈 0. 05）. IL-6 and MMP-9 in the experimental group were significantly lower than those in the control group （ P 〈 0.05 ）. The heart/body weight ratio in the experimental group was significantly lower than that in the control group （P 〈 0. 05 ）. Pathological examination showed that there were significant hypertensive and inflammatorg changes in the kidney, heart and aorta of the control group, while the experimental group was much better. Conclusions Regular administration of vitamin D3 medication can regulate the blood pressure by suppressing inflammatory factors IL-6 and MMP-9 and inhibiting inflammatory response in the hypertensive rats.