补佳乐联合缩宫素建立小鼠原发性痛经模型

Establishment of a mouse model of primary dysmenorrhea model with progynova and oxytocin

目的建立小鼠原发性痛经模型。方法不同剂量补佳乐给近交系BALB/c小鼠连续灌胃,末次给药后腹腔注射缩宫素,诱发扭体反应,记录扭体潜伏期和扭体次数,筛选最佳条件。结果补佳乐最佳剂量为0.5 mg/kg;催产素最佳剂量为每只2 U;补佳乐灌胃后1 h为观察扭体反应的最佳时间,用药周期第3天时扭体次数开始增多;从第4天开始维持在高水平。结论补佳乐联合缩宫素可以成功建立小鼠原发性痛经模型。

Objective To establish a mouse model of primary dysmenorrhea with progynova and oxytocin. Methods In this study 6- to 8-week old female Balb/c mice were used. To administer different doses of progynova to mice continuously and after the last dosage peritoneally to inject oxytocin to induce writhing response. To record the writhing latency period and frequency. Results The optimal dose of progynova was 0.5 mg/kg and oxytocin 2 U/mouse. One hour after administration of progynova was the optimal time to observe writhing response. From the third day writhing frequency began to increase and maintain a high level from the fourth day. Conclusion An animal model of primary dysmenorrhea can be successfully established in mice with progynova in combination with oxytocin.