模拟航天特因环境下大鼠认知功能的影响

Influence of simulated spaceflight environment on cognitive functions in rats

目的采用行为学方法研究拟航天复合特因环境下大鼠认知功能的影响。方法以尾吊、隔离、孤养和昼夜节律改变等复合应激为建模条件,分别于14、28 d,采用奖励性条件反射和水迷宫检测认知功能。结果奖励性条件反射中,14 d时,与正常组相比,复合环境组鼻触次数减少(P<0.05);28 d时,复合环境与正常组和尾平组间形成显著性差异(P<0.01)。水迷宫测试中,14 d时,复合环境组与正常组和尾平组相比,逃避潜伏期和总游程均有不同程度的增加(P<0.01,P<0.05);28d时,差异进一步增大(P<0.01)。正常组与尾平组间无差异。结论航天复合环境模型可致大鼠认知功能严重损伤,28 d时,认知功能损伤模型较为稳定。

Objective Using behavioral testing to study the influence of simulated spaceflight environment on cognitive function in rats. Methods Tail suspension, separation, isolation and circadian rhythm changes were used to simulate the spaceflight environment. Reward conditioning test and Morris water maze （MWM） test were used to determine the cognitive function of rats after 14 days and 28 days in the simulated compound spaceflight environment. Results Upon reward conditioning test, compared with the control （Con） group, the rats of the compound environment （CE） group showed lower number of nose pokes （P 〈 0.05） at 14 days. The number of nose pokes in the CE group at 28 d was significantly less than that in the control group and tail restraint （TR） group （P 〈 0.01 ）. Upon the Morris water maze （MWM） test, compared with the control group and TR group, the CE group exhibited longer escaping latency and total distance at 14 d （P〈0.01 ,P 〈0.05）. At 28 d, the difference became larger （P 〈0.01）. The control group and TR group were not significantly different in reward conditioning test and MWM test. Conclusions Simulated compound spaceflight environment can induce serious cognitive deficit, and the models of cognitive deficit are more stable at 28 days.