应用过氧化氢发生器对实验动物屏障环境及设施消毒效果的观察

Observation on sterilization effects of hydrogen peroxide vapor in laboratory animal barrier environment

目的观察过氧化氢(H2O2)蒸汽对屏障环境内表面、台面以及空气的消毒效果。方法用BIOQUELL Z过氧化氢气体发生器将浓度为30%的H2O2液体分别按6 mL/m3、9 mL/m3和12 mL/m3的用量进行汽化熏蒸处理,作用60 min,检测屏障环境空气落下菌数和表面细菌数。结果 6 mL/m3用量能够杀灭二更、清洁走廊、洁存间、次清洁走廊和缓冲间等空气落下菌较少区域的空气细菌;9 mL/m3用量可以完全杀灭动物饲养室的空气细菌,但不能完全杀死动物饲养室内表面细菌;而12 mL/m3用量可以完全杀死动物饲养室内表面细菌。结论H2O2蒸汽消毒适合屏障环境微生物的控制,操作简单,效果明显。

Objective To observe the sterilization effects of hydrogen peroxide vapor on hard surface and air in laboratory animal barrier environment. Methods BIOQUELL Z sterilizer continuously sprayed 30% hydrogen peroxide vapor by 6 mL/m^3 , 9 mL/m^3 and 12 mL/m^3 for 60 min at different areas in laboratory animal barrier environment. The numbers of failing bacteria and bacteria on the surface in barrier environment were detected. Results The falling bacteria in second changing rooms, clean corridor, storage room, dirt corridor and buffer room were killed completely by amount of 6 mL/m^3 H2O2, and the falling bacteria in animal feeding room were killed completely by amount of 9 mL/m^3 H202 , but the bacteria on the surface in barrier environment were not completely killed. The bacteria on the surface in barrier environment were completely killed by amount of 12 mL/m^3 H2O2. Conclusion Hydrogen peroxide vapor has very good sterilization effects on laboratory animal barrier environment and housing facilities.