Toll样受体激动剂不同给予时间对P.y17XL感染BALB/c小鼠原虫血症及存活率的影响

Influence of different given time of toll-like receptor agonists on the development of Plasmodium yoelii 17XL and the life span of infected mice

目的观察Toll样受体(TLR)激动剂不同给予时间对约氏疟原虫17XL(P.y17XL)感染BLAB/c小鼠原虫血症及存活率的影响。方法BALB/c小鼠分为6组,每组5只,感染前(D-1)和后(D+1)24 h分别尾静脉注射20μg TLR3激动剂Poly(I:C)和10μg TLR7激动剂R837(以注射或未注射200μl PBS组为阴性对照),然后每只小鼠腹腔注射2×105P.y17XL,感染后隔天查小鼠的原虫血症,记录原虫血症和存活率。结果与对照组小鼠比较,给予Poly(I:C)D-1组生存期缩短,原虫血症较野生株小鼠高,差异有统计学意义(P<0.05),而给予TLR7激动剂R837组,无论是D-1或D+1组,其生存期均延长,原虫血症较野生株小鼠低,差异有统计学意义(P<0.05)。结论 TLR3激动剂Poly(I:C)D-1能够促进P.y17XL的增殖,降低感染小鼠的生存期;而TLR7激动剂R837无论是D-1或D+1给予均能抑制P.y17XL的增殖,并能延长感染小鼠的存活时间。

Objective To investigate the effect of TLR3 and TLR7 agonist on the development of P.y17XL and the mortality of P.y17XL-infected BALB/c mice. Methods 6 groups of normal BALB/c mice were used. 20 μg TLR3 agonist Poly （I：C） and 10 μg TLR7 agonist R837 were intravenously injected 24 h before and after P.y17XL infection. The survival rate of the mice and P.y17XL parasitemia were observed at the interval day after intraperitoneal injection of 2×10^5 of P.y17XL. Results Compared with the control mice group, the life span of the experimental group, which was given the TLR3 agonist Poly （I：C） before infection, was shortened and the degree of parasitemia was slightly higher（P〈 0.05）. However,mice which receive the TLR7 agonist R837 at any time can extend its lifetime and reduce the parasitemia. Conclusion TLR agonist may affect the development of P.y17XL and change the life span of P.y17XL- infected mice.