杨梅素减轻高糖诱导的血管内皮细胞氧化应激损伤

Myricetin attenuates high glucose-induced oxidative stress in vascular endothelial cells

目的探讨杨梅素对高糖诱导的血管内皮细胞氧化应激损伤的影响。方法体外培养原代人脐静脉内皮细胞（human umbilical vein endothelial cells,HUVECs）,分为正常对照组（葡萄糖浓度5.56 mmol/L）、甘露醇高渗对照组（5.56 mmol/L葡萄糖＋27.8 mmol/L甘露醇）、高糖处理组（葡萄糖浓度33.36 mmol/L）、杨梅素保护组（0.1~10μmo/L杨梅素＋33.36 mmol/L葡萄糖）,处理48 h后,CCK-8比色法检测细胞活力,Annexin-FITC/PI双染流式细胞术检测细胞凋亡,同时检测细胞内活性氧（ROS）含量,及培养上清液中丙二醛（MDA）含量、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性。结果高糖处理HUVECs 48h后,与正常对照组比较,细胞活力显著降低（P〈0.05）,细胞凋亡率、ROS及MDA含量均显著增加（P〈0.05）,而SOD和CAT活性显著下降（P〈0.05）;杨梅素预处理HUVECs后,可显著抑制高糖引起的HUVECs细胞凋亡、ROS和MDA含量的增加以及细胞活力、SOD和CAT活性的下降（P〈0.05）。结论杨梅素可减轻高糖诱导的血管内皮细胞氧化应激,抑制细胞凋亡。

Objective To determine the effects of myricetin on oxidative stress in vascular endothelial cells induced by high glucose.Methods Human umbilical vascular endothelial cells（HUVECs） were treated by 5.56 mmol/L glucose as normal control,5.56 mmol/L glucose plus 27.8 mmol/L mannitol as an osmotic control,or 33.36 mmol/L glucose for 48 h in the absence and presence of myricetin（0.1 to 10 μmol/L）.Cell viability and apoptosis were detected by CCK-8 assays and annexin V-FITC/PI double staining using flow cytometry,respectively.In addition, the intracellular reactive oxygen species（ROS）, malondialdehyde（MDA） content and the activities of superoxide dismutase（SOD） and catalase（CAT） in the culture supernatants were measured.Results Compared with the normal control group,high glucose significantly decreased cell viability and the activities of SOD and CAT（P0.05）,while increased the apoptotic rate,intracellular ROS and MDA contents in HUVECs（P0.05）.However,myricetin pretreatment significantly inhibited these high glucose-induced above phenomena（P0.05）.Conclusion Myricetin attenuates high glucose-induced oxidative stress and apoptosis in vascular endothelial cells.