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基质辅助激光解吸电离飞行时间质谱技术在快速检测病原菌中的研究进展 被引量：2

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摘要 传统的临床微生物学检验依赖于长时间的对病原菌的分离、培养及复杂的生化鉴定,有些病原微生物的生化反应不典型则难以准确鉴定。分子生物学技术如PCR技术要求较高,常规实验室不易开展。基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)作为一种跨学科的检测新技术,在细菌和真菌的快速鉴定、细菌的耐药性检测、流行病学分析方面呈现出较传统鉴定法较多的优势。
作者 王沛 杨燕妮
机构地区 荆门市第一人民医院检验科
出处 《临床检验杂志》 CAS CSCD 北大核心 2013年第9期678-680,共3页 Chinese Journal of Clinical Laboratory Science
关键词 基质辅助激光解吸电离飞行时间质谱 常见病原菌 临床检测
分类号 R466.5 [医药卫生—临床医学]
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参考文献20

  • 1Wemo AM, Christner M, Anderson TP, et al. Differentiation of Streptococcus pneumoniae from nonpneumococcal streptococci of the Streptococcus mitis group by matrix-assisted laser desorption ioniza- tion-time of flight mass spectrometry [ J ]. J Clin M icrobiol, 2012, 50 (9) :2863-2867.
  • 2Seng P, Drancourt M, Gouriet F, et al. Ongoing revolution in bacte- riology: routine identification of bacteria by matrix-assisted laser de- sorption ionization time-of-flight mass spectrometry[J] Clin Infect Dis, 2009, 49 (4) : 543-551.
  • 3Schaumann R, Knoop N, Genzel GH, et al. Discrimination of Enter- obacteriaceae and non-fermenting gram negative bacilli by MALDI- TOF moss spectrometry[ J]. Open Microbiol J, 2013, 7 : 118-122.
  • 4Bader O, Weig M, Taveme-Ghadwal L,et al. Improved clinical labo- ratory identification of human pathogenic yeasts by matrix-assisted la- ser desorption ionization time-of-flight mass spectrometry [ J ]. Clin Microbiol Infect, 2011, 17(9) : 1359-1365.
  • 5Hof H, Eigner U, Maier T, et al. Differentiation of Candida dublini- ens/s from Candida albicans by means of MALDI-TOF mass spectrom- etry[J]. Clin Lab, 2012, 58(9-10) :927-931.
  • 6Putignani L, Del Chierico F, Onori M,et al. MALDI-TOF mass spec- trometry proteomic phenotyping of clinically relevant fungi [ J ]. Mol Biosyst, 2011, 7(3 ): 620-629.
  • 7Haigh J, Degun A, Eydmann M, et al. Improved performance of bac- terium and yeast identification by a commercial matrix-assisted laser desorption ionization-time of flight mass spectrometry system in the clinical microbiology laboratory [ J ]. J Clin Microbiol, 2011,49 (9) : 3441.
  • 8Majcherezyk PA, MeKenna T, Noreillon P,et al. The discriminatory power of NALDI-TOF mass spectrometry to differentiate between iso- genie teicoplanin-susceptible and teieoplanin-resistant strains of me- thieillin-resistant Staphylococcus aureus [ J ]. FENS Microbiol Lett, 2006, 255(2) :233-239.
  • 9孙宗科,张伟,陈西平.基质辅助激光解析电离飞行时间质谱快速鉴定耐甲氧西林金黄色葡萄球菌[J].卫生研究,2011,40(3):375-378. 被引量：9
  • 10Burckhardt I, Zimmermann S. Using matrix-assisted laser desorption ionization-time of flight mass spectrometry to detect carbapenem re- sistance within 1 to 2.5 hours[ J]. J Clin Microbiol, 2011,49 (9) : 3321-3324.

二级参考文献22

  • 1MANDELL G,DOUGLAS J,BENNETT R. Principles and practices of infectious diseases [ M ]. 4th ed. Edinburgh, United Kingdom: Churchill Livingstone, Ltd, 1995.
  • 2National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. NCCLS document MT-A4 [ S ]. Wayne, Pa : National Committee for Clinical Laboratory Standards, 1997.
  • 3ANHALT J P, FENSELAU C. Identification of bacteria using mass spectrometry[J].Anal Chem, 1975,47:219-225.
  • 4HELLER D N, FENSELAU C, COTTER R J, et al. Mass spectral analysis of complex lipids desorbed directly from lyophilized membranes and cells [ J ]. B iochem Biophys Res Commun, 1987,142: 194-199.
  • 5HELLER D N, COTTER R J, FENSELAU C, et al. Profiling of bacteria by fast atom bombardment mass spectrometry [ J ]. Anal Chem, 1987,59 : 2806-2809.
  • 6HOB C, FENSELAU C, HANSEN G, et al. Dipalmitoylphosphatidylc holine in amniotic fluid quantified by FAST-atom-bombardment mass spectrometry [ J ]. Ella Chem, 1983,29 : 1349-1353.
  • 7CLADON M A, DAVEY S N, EDWARD-JONES Y, et al. The rapid identification of intact microorganisms using mass spectrometry [J]. Nat Biotechnol, 1996,14 : 1584-1586.
  • 8HOLLAND R D,WILKES J G, RAFII F,et al. Rapid identification of intact whole bacteria based on spectral patterns using matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry[J]. Rapid Commun Mass Spectrom, 1996,10 : 1227-1232.
  • 9KRISHNAMURTHY T, ROSS P L, RAJAMANI U. Detection of pathogenic and non-pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [J]. Rapid Commun. Mass Spectrom, 1996,10:883-888.
  • 10HACHBARTH C J, CHAMBERS H F. Methicillin-resistant staphylococcus: genetics and mechanisms of resistance [J].Antimicrob Agents Chemother, 1989,33 (7) :991-994.

共引文献17

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二级引证文献16

临床检验杂志
2013年 第9期

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