芥菜在聚乙二醇模拟干旱条件下的生理变化研究

Study on Physiological Changs of Brassica juncea Coss.Under Drought Stress Simulated by PEG6000

以云南地方芥菜品种"M100"(Brassica juncea Coss.)为试材,研究了5%、8%和10%聚乙二醇(PEG6000)渗透胁迫下芥菜幼苗的叶绿素含量、丙二醛(MDA)含量、脯氨酸含量、可溶性糖含量、相对含水量、相对电导率和Fv/Fm等生理指标随胁迫处理时间的变化。结果表明:随着PEG6000胁迫时间的延长或PEG6000浓度的升高,芥菜叶片叶绿素含量、相对含水量、Fv/Fm下降比较明显,MDA含量、脯氨酸含量、可溶性糖含量和相对电导率则表现出不同程度的增加趋势。其中MDA含量、脯氨酸含量、可溶性糖含量、相对含水量和相对电导率5个指标在PEG6000浓度为10%时,各处理间差异显著,因此,这5个指标可用于测定芥菜的耐旱性。

Taking Yunnan local mustard variety ＇M100＇ （Brassica juncea Coss. ） as test material, the physiological changes under PEG6000 stress with the concentrations of 5% ,8% and 10 % were investigated. Physiological indexes such as the content of chlorophyll, MDA, proline, soluble sugar, relative water content, relative electric conductivity, Fv/Fm were determined with the prolongation of the time of PEG6000-induced stress. The results showed that with the increasing of PEG6000 concentration and the prolongation of treatment time, chlorophyll, relative water content, Fv/Fm of the leaf decreased obviously,and the content of MDA,proline,soluble sucrose,relative electric conductivity increased in varying degrees. Among these indexes, MDA content, proline content, soluble sugar content, relative water content, relative electric conductivity reached a significant difference between treatments under the concentration of 10% PEG6000 simulated drought stress, so these five indexes could be used for determination of mustard drought resistance.