三个黄伞菌株不同生长发育期几种胞外酶活性变化

Activity Changes of Several Extracellular Enzymes from Three Pholiota adipose Strains at Different Stages of Development

以3个黄伞菌株为试材,以棉籽壳为主料,采用瓶栽方法对其不同生长发育期的几种胞外酶活性进行了测定,以期揭示同种食用真菌不同菌株间的相同胞外酶活性变化特点。结果表明:3个黄伞菌株均具有完整的胞外纤维素酶系;3个黄伞菌株的胞外羧甲基纤维素酶(CMC酶)、滤纸纤维素酶(FP酶)、半纤维素酶(CH酶)、淀粉酶和果胶酶活性高峰均对应于子实体生长发育期;在整个栽培期,3个黄伞菌株的胞外β?葡萄糖苷酶均无活性高峰出现;3个黄伞菌株的漆酶在菌丝满瓶期和潮菇间期均有活性高峰出现,在头潮菇和2潮菇子实体成熟期,漆酶活性均处于较低水平;3个黄伞菌株的胞外过氧化物酶在菌丝生长期均具有较高活性,头潮菇原基期后,过氧化物酶活性均显著下降。可见3个黄伞菌株的相同胞外酶的活性变化趋势大致相同。

Taking three Pkoliota adipose strain as materials, the activity changes of several extracellular enzymes were determined at different stages of development from three Pholiota adipose strains cultivated on cotton seed hull in bottle to reveal the features of activity changes of the same extracellular enzymes from different strains of the same edible fungi. The results indicated that all of the three strains had complete set of extracellular cellulose; the activity peaks of extracellular carboxymenthel cellulase （CMCase）, filter paper cellulase （FPase）, hemicellulase （HCase）, amylase and pectase from the three strains all appeared in the period between primordial formation and fruit-body maturity;there were no great changes in 13-ghlcosidase activity from the three strains throughout its growth;the activity peaks of cxtracellular laccase from the three strains appeared in the period when substrates were fully colonized and in the period hetween first and second flush but their activities were low in the fruit-body maturity of both the first and second {lush; and the extracellular peroxidase activities from the three strains remained high in the period of mycelial growth and then declined rapidly at the beginning of fruiting. It could be concluded that the activity changes of the same extracellular enzymes from lhe three strains were roughly the same.