药用植物龙葵内生真菌分离及其抑菌活性初探

Study on the Isolation of Endophytic Fungus fromSolanum nigrumL.and Its Microbial Inhibition Activity

以药用植物龙葵为试材,通过组织块法对其内生真菌进行分离,并选择5种植物病原真菌和4种细菌作为指示菌,采用平板对峙法和改进的菌块法测定抑菌活性以研究内生真菌多样性及其抑菌活性特征。结果表明:从龙葵根、茎、叶分离出29株内生真菌,其中根部最多;经形态学初步分类鉴定归于2目2科4属。79.3%的株菌对2种或多种植物病原真菌有不同程度的抑制作用,41.4%的株菌对1种或多种细菌指示菌具有不同程度的抑制作用;菌株‘SNEFL012’对4种病原真菌具有明显抑制作用,菌株‘SNEFS005’对2种细菌和2种真菌指示菌具有明显抑制作用,菌株‘SNEFL012’和‘SNEFS005’分属于曲霉属和梭孢霉属。药用植物龙葵具有较为丰富的抑菌活性内生真菌资源,其抑菌活性值得进一步研究。

Taking medicinal plant Solanum nigrum L. as material,the endophytic fungi strains were isolated from root, stem and leaf of indicators to test order to explore 1hat twenty-nine the host by lissue inoculation culture and five pathogerfic fungi and four bacteria strains were used as microbial inhibition activities by agar plate antagonistic action and modified agar gel diffusion methods in the resource diversity and microbial inhibition activity of endophytic fungi from it. The results indicated fungal endophytic strains were isolated from the host,most of them coming from root,then from leaf and stem; the isolated strains attributed to four genera, two families, and two orders based on morphological characteristics; 79.3% of the total isolated strains were found to have some microbial inhibition activities against two or more indicating fungi,41.4% of the total isolates had antibacterial activities against one or more indicating bacteria; the strain ＇SNEFL012＇ had evident inhibition activities to four kinds of pathogenic indicators, and the strain ＇SNEFS005＇ had evident inhibition activities to two kinds of bacterial indicators and two kinds of pathogenic indicators, the two bioactive strains with evident antimicrobial activities belonging to plant Solanum nigrum L. showed relatively abundant microbial inhibition was worthwhile for further studies. Aspergillus sp. and Fusidium sp. , respectively. The medicinal endophytic fungal resource for pathogenic inhibition, and its microbial inhibition was worthwhile for further studies.