3种木通属植物的AFLP研究

AFLP Analysis of Three Species from Akebia Genus

目的:构建木通属植物的DNA指纹图谱,探讨利用扩增片段长度多态性(AFLP)分子标记在木通属植物遗传多样性、亲缘关系及种质鉴别上的可行性。方法:采用AFLP技术,随机选用8对EcoRⅠ/MseⅠ引物组合构建样品DNA指纹图谱。结果:从64对引物中筛选出带型丰富、多态性较高的8对引物,共检测到977条多态性条带,多态性比率达92.61%。遗传相似系数以及UPGMA聚类分析结果表明:在遗传相似系数0.49处,12个供试样品分为2类:木通为一类,白木通和所有三叶木通聚为一类;此与基于形态学方面的分类结果一致。结论:AFLP技术适用于木通属植物的亲缘关系及种质鉴别分析。

Objective ： To establish the DNA fingerprints of three species from Akebia genus markers and find the feasibility in analyzing their genetic diversity, relationship and germplasm identification by Amplified Fragment Length Polymorphsm（AFLP）. Methods： The DNA polymorphism of 12 samples from three species of Akebia genus were detected by AFLP molecular markers. Results：Among 64 primer pairs ,8 were found to yield rich band patterns and high po]ymorphism. After amplification with these 8 primer pairs to 12 sampies, a total of 977 polymorphic DNA bands were detected. The polymorphism rate reached 92. 61%. The coefficient of genetic similarity and UPGMA clustering analysis were consistent with the classification based on morphological observations. The coefficient of genetic similarity was 0.49 and the 12 samples could be clustered into 2 groups. Akebia quinata samples formed a single cluster and Akebia trifoliata var. australis and Akebia trifoliata samples formed another cluster. Conclusion ： AFLP markers can be potentially used in analyzing of genetic diversity, relationship and germplasm identification of these medicinal plants.