过氧化氢-碘化物-吖啶橙体系共振散射法测定痕量过氧化氢

Determination of trace hydrogen peroxide by resonance light scattering in the system of hydrogen peroxide-iodide-acridine orange

在pH4．5的柠檬酸-柠檬酸钠缓冲液中，牛血红蛋白催化H2O2氧化I-产生I3-，I33-与吖啶橙进一步发生反应形成离子缔合物，导致体系共振散射强度明显增大，据此提出了一种测定痕量H：02的新方法。在528．95nm处，H202浓度在4．12×10^-7-1．65×10^5mol／L范围内与共振散射强度的改变值△I呈良好线性关系，检出限为3．23×10^-8mol／L。方法已用于水样中H2O2的测定。

A new resonance light scattering （RLS） method for the determination of hydrogen peroxide was developed. The method was based on the reaction of hydrogen catalytic effect of bovine hemoglobin in citric acid-sodium citrate with acridine orange （AO） to form an ion association complex, peroxide with excess I- to produce 13 - by the buffer solution of pH4. 7, and 13 - further reacted resulting in great enhancement of the intensity of RLS of the system. At the maximum RLS wavelength of 528.95 rim, the enhanced RLS intensity of the system was linearly related to the concentration of hydrogen peroxide in the range of 4. 12 × 10^-7 -1.65 × 10^-5 mol/L, the detection limit was 3.23 × 10^-8 mol/L. This method has been applied to the determination of hydrogen peroxide concentration in water samples with satisfactory results.