反相高效液相色谱法同时测定果蔬汁饮料中的11种酚酸化合物

Simultaneous determination of 11 phenolic acids in fruit and vegetable juice drinks by reversed phase high performance liquid chromatography

建立了同时测定果蔬汁饮料中11种酚酸化合物的反相高效液相色谱方法。采用WondaSilC18色谱柱（4．6mm×250mm，5μm），流动相为乙腈和体积分数0．5％乙酸水溶液，梯度洗脱，柱温35℃，流速1．0mL／min，波长为280nm和330nm。在1．0—50．0mg／L的线性范围内，11种酚酸的相关系数均大于0．999，相对标准偏差均小于2％，回收率为90．2％～102．2％，检出限为0．015～0．030mg／L。

A method of simultaneous determination of 11 phenolic acids in fruit and vegetable juice drinks by reversed phase high performance liquid chromatography was established. Separation was performed on a WondaSil Cis column （4. 6 mm ×50 mm,5 μm） at 35 ℃y gradient elution. The mobile phase consisted of acetonitrile and water with 0. 5% acetic acid at a flow rate of 1.0 mL/min. The detection wavelengths were 280 nm and 330 nm. The linears ranges of phenolic acids were in the range of 1.0 ~ 50. 0 mg/L. The correlation coefficients for the regression equations were all higher than 0. 999 when relative standard deviations were all less than 2%. The recoveries for the phenolic acids determined were in the range of 90. 2%- 102. 2% and the detection limit of the method was ranged from 0. 015 to 0. 030 mg/L.