硫酸镁抗急性肺动脉高压的炎症机制

nti-inflammation of MgS04 on acute pulmonary hypertension

目的探讨硫酸镁（MgSO4）对急性肺栓塞肺动脉高压的影响及其抗炎机制。方法30只雄性杂种犬，体质量（15±2．1）kg，随机分为5组：空白组、对照组以及MgSO47．0组、MgSO48．0组、MgSO49．0组，每组6只，其中空白组不给予任何处理；对照组造成急性肺动脉高压；其余三组造成急性肺动脉高压后给予MgSO4静滴，稳定30min后，MgSO4 200mg／kg静脉滴注0．5h，然后维持血MgSO4浓度分别为7．0mmol／L、8．0mmol／L以及9．0mmol／L。肺动脉、股动脉插管检测平均肺动脉压（MPAP）、体循环平均动脉压（MAP），应用酶联免疫吸附以及放免法分别测定血清中白介素6（IL-6）和肿瘤坏死因子a（TNF-a），Westernblot测定肺组织中单核细胞趋化蛋白-1（MCP-1）蛋白表达。结果①对照组MPAP较空白组显著升高（P〈0．01），MgSO 47．0组、MgSO48．0组以及MgSO4 9．0组，MPAP较对照组显著下降（P〈0．05或P〈0．01），其中MgSO49．0组下降最明显；对照组MAP较空白组显著降低（P〈0．05或P〈0．01），MgSO47．0组、MgSO4 8．0组以及MgSO49．0组MAP较对照组显著升高（P〈0．05或P〈0．01），其中MgSO49．0组升高最明显。②对照组血清中TNFa以及IL-6较空白组升高（P〈0．05），MgSO47．0组、MgSO48．0组以及MgSO49．0组TNF-α以及IL-6较对照组显著下降（P〈0．05或P〈0．01），MgSO49．0组下降最明显；③对照组MCP-1蛋白表达较空白组显著升高（P〈0．01），MgSO4 7．0组、MgSO48．0组以及MgSO49．0MCP-1蛋白表达较对照组显著下降（P〈0．01），MgSO4 9．0组蛋白表达最低。结论MgSO4可显著降低肺动脉高压，这可能与其抑制肺组织MCP-1表达，降低TNF-α以及IL-6，从而抑制炎症反应增强有关。

Objective To investigate the effect and the anti-inflammatory mechanism of magnesium sulfate （MgSO4） on acute pulmonary hypertension. Methods 30 mongrel dogs were randomly divided into five groups： control group, surgical group, MgSO4 7.0 group, MgSO4 8.0 group, and MgSO4 9.0 group. The control group was not given any treatment, and the surgical group was operated to induce acute pulmonary hypertension,and the three MgSO4 groups were given MgSO4 to maintain intravenous concentration with 7.0 mmol/L, 8.0 mmol/L, and 9.0 mmol/L respectively after acute pulmonary hypertension. The mean pulmonary artery pressure （MPAP） and circulation mean arterial pressure （MAP） were detected. Interleukin-6 （IL-6） and tumor necrosis factor-a （TNF-6） in serum were measured by enzyme-linked immunosorhent assay and radioimmunoassay, monocyte chemoattractant protein-1 （MCP-1） expression in the lung tissue was determined by western blot. Results O MPAP,TNF-a, IL-6, and MCP-1 protein expression were significantly higher in surgical group than those in control group,and were decreased significantly in MgSO4 7.0 group, MgSO4 8.0 group, and MgSO4 9.0 group with dose dependence. （1）MAP was significantly lower in surgical group than that in control group. MAP in MgSO4 7.0 group, MgSO4 8.0 group,and MgSO4 9.0 group was significantly increased compared with surgical group and MAP in MgSO4 9.0 group was the highest. Conclusions MgSO4 significantly reducespulmonary hypertension, which is probably associated with reduction of the inflammatory response by inhibition of MCP 1 expression in lung tissue and decrease of TNF a and IL-6 in serum.