卡麦角林处理哺乳期小鼠对子代存活和生长的影响

Effects of cabergoline in lactating mice on survival and body growth of their pups

哺乳期是兽类繁殖成功的关键阶段之一,本实验以不同浓度卡麦角林油溶液对哺乳早期雌性小鼠连续灌胃3 d,检测雌性小鼠体重和幼鼠存活及体重变化,以此确定卡麦角林对哺乳雌性小鼠繁殖成功的影响。结果发现,卡麦角林对雌性小鼠在哺乳期的体重相对增长率无显著影响,但处理组幼鼠出现部分死亡,0μg/kg、50μg/kg、100μg/kg、200μg/kg、400μg/kg组幼鼠死亡率分别为1.30%、3.85%、4.08%、7.38%、21.19%,幼鼠死亡数和剂量呈正相关,400μg/kg组幼鼠成活率显著下降到78.8%,其他剂量组幼鼠成活率与对照组差异不显著;给药后7 d存活幼鼠的生长发育显著被抑制,之后处理组幼鼠体重变化与对照组无显著差异。上述结果表明卡麦角林处理哺乳早期雌性小鼠可降低幼鼠成活率并抑制存活幼鼠的生长发育,进而有可能降低雌性小鼠及其子代的繁殖成功率。

To select the contraceptive which inhibited reproduction of mice during lactation, we administered different do- ses of eabergoline in oil to female mice by gastrogavage on days 1, 2, and 3 of lactation; we recorded the survival and mortality of pups as well as the body masses of the female mice and their pups. Thus, we could confirm the effects of cabergoline treatment on lactating mice and the development of their pups during lactation. Results showed that cabergoline treatment had no significant effect on the relative growth rate of mice during lactation. Some pups in the treated group died. The mortality rates were 1.30%, 3.85%, 4. 08%, 7.38%, 21.19% in the groups that were administered with 0 μg/kg, 50 μg/kg, 100 μg/kg, 200 μg/kg, and 400 μg/kg doses of eabergoline, respectively. Thus, the mortality of pups and the dose of cabergoline were positively related. We also found that cabergoline could reduce the survival rate of the pups. In particular, the survival rate was significantly reduced to 78. 8% in the group treated with 400 p,g/kg cabergo- line compared with that for the control group. The survival rate of other groups were not significantly different from that of the control group. Cabergoline treatment significantly inhibited the development of pups for up to 7 d. Afterward, their development was similar to that of the control group. Thus, cabergoline could decrease the survival rate of pups, inhibit the development of the surviving pups, and probably interfere with the reproductive success of the mice and their pups.