心脉康胶囊对大鼠缺血再灌注所致心肌梗死的作用机制研究

Action mechanism of Xinmaikang capsules in rats with myocardial infarction caused by ischemia/reperfusion

目的:探讨心脉康胶囊对大鼠缺血再灌注所致心肌梗死的作用机制。方法:将48只雄性Wistar大鼠随机分为假手术组、模型组、合心爽组和心脉康高、中、低剂量组,每组8只。对除假手术组的另外5组大鼠进行缺血再灌注所致心肌梗死模型制备。通过十二指肠给药,合心爽组剂量为16mg/kg体质量,心脉康高、中、低剂量组给药剂量分别为4.0、2.0、1.0g/kg体质量,给药体积为3mL/kg,假手术组和模型组不予给药。各组大鼠恢复自主呼吸2h后,经腹主动脉取血,用比色法测定其血清超氧化物歧化酶(SOD)和丙二醛(MDA)含量;采用多媒体彩色病理图文分析系统,以固定象距测量正常心肌与梗死心肌面积,观察心肌梗死程度。结果:心脉康胶囊各剂量组能明显减轻大鼠缺血再灌注所致心肌梗死程度,缩小梗死面积,减轻梗死区重量,同时增加SOD活性,与模型组比较,差异均有统计学意义(P<0.05,P<0.01);各药物组MDA含量与模型组比较无显著变化。结论:心脉康胶囊对大鼠心肌缺血再灌注损伤的保护作用可能是通过抑制脂质过氧化、减少氧自由基对心肌的损伤来实现的。

Objective ：To investigate the action mechanism of Xinmaikang capsules in rats with myocardial infarction caused by is- chemia/reperfusion. Methods ： Forty - eight male Wistar rats were randomly and equally divided into sham - operation group, model group, diltiazem group, and high - , middle - , and low - dose Xinmaikang groups. A rat model of myocardial infarction was established by ischemia/reperfusion in all groups except sham -operation group. The dihiazem group received diltiazem （16 mg/kg body weight） ; the high- , middle- , and low- dose Xinmaikang groups received Xinmaikaug capsules （4. 0,2.0, and 1.0 g/kg body weight, respec- tively;3 ml/kg） ;the sham- operation group and model group received no medication. Medication was given via the duodenum. At 2 h after spontaneous breathing was restored in all groups, blood samples were taken from the abdominal aorta. Superoxide dismutase （SOD） activity and malondialdehyde （MDA）content in serum were determined by colorimetry. The areas of normal myocardium and infarcted myocardium were measured using the multimedia pathological image and word analysis system at a fixed image distance, and the severity of myocardial infarction was evaluated accordingly. Results：Compared with the model group, all Xinmaikang groups had significantly reduced myocardial infarction caused by ischemia/reperfusion, significantly decreased infarct area and infarct weight, and significantly increased SOD activity （P 〈0. 05 or P 〈 0. 01 ）. There were no significant differences in MDA content between all medication groups and the model group. Conelusion：Xinmaikang capsules can protect rats from myocardial ischemia- reperfusion injury ,which may be a- chieved by inhibiting lipid peroxidation and reducing myocardial damage caused by oxygen radicals.