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DNA甲基化标记检测方法及其法医学应用 被引量：6

DNA methylation detection technologies,and their prospects for forensic applications
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摘要 DNA甲基化是重要的表观遗传修饰,主要在转录水平上调控基因的表达。DNA甲基化在人类基因组中含量丰富、分布广泛;甲基化谱有时空特异性、细胞特异性和亲源特异性。新近研究表明DNA甲基化在组织体液鉴定、同卵双生子鉴定、年龄推断、性别推断和亲子鉴定等方面具有一定的应用价值,有望成为一种新的法医学遗传标记。本文就DNA甲基化检测方法的研究进展及法医学应用进行综述,以期为法医学实践提供参考。 As an important epigenetic marker, DNA methylation is abundance and widely distributed in the human genome, and plays a key role in regulation of gene expression at the transcriptional level. DNA methylation possesses timing- and tissue- specific expression and parental specificity in genome. It is reported that the DNA methylation could be used as a novel genetic marker in the field of forensic science, including body fluid identification, monozygotic twins discrimination, estimation of age, and sex and parentage testing. This paper is aimed to make a brief review on the detection methodologies of DNA methylation as well as its applications in forensic practice.
作者 周娣 王正 张霁 侯一平
机构地区 四川大学华西基础医学与法医学院法医遗传教研室
出处 《中国法医学杂志》 CSCD 2013年第5期394-397,共4页 Chinese Journal of Forensic Medicine
关键词 法医物证学 体液鉴定 DNA甲基化 焦磷酸测序 forensic genetics body fluid identification DNA methylation pyrosequencing
分类号 D919 [医药卫生—法医学]
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参考文献19

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二级参考文献35

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共引文献27

同被引文献44

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引证文献6

二级引证文献12

中国法医学杂志
2013年 第5期

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