常见水产药物对两种水产益生菌活性的影响

Effects of Different Fishery Drugs on Activity of Denitrifying Bacteria Pseudomonas stutzeri and Bacillus amyloliquefaciens

为探求水产药物影响反硝化细菌和芽孢杆菌组成的微生态制剂的水质净化效果,研究了高锰酸钾、硫酸铜合剂、二氧化氯、戊二醛和阿维菌素对反硝化细菌和芽孢杆菌生长和脱氮的影响。试验结果表明,反硝化细菌对高锰酸钾、戊二醛和二氧化氯较为敏感,高锰酸钾质量浓度为4mg/L,戊二醛质量浓度为0.1mg/L时,反硝化细菌对亚硝酸盐氮的去除率显著降低(P<0.05),二氧化氯质量浓度为0.3mg/L时,反硝化细菌的生物量和脱氮能力显著降低(P<0.05),在实际应用中应避免混合使用;而反硝化细菌对硫酸铜合剂、阿维菌素不敏感,可以混合使用;芽孢杆菌对高锰酸钾,硫酸铜合剂,二氧化氯较为敏感,高锰酸钾质量浓度为2mg/L,硫酸铜合剂质量浓度为0.25mg/L,二氧化氯质量浓度为1mg/L时,芽孢杆菌的对亚硝酸盐氮的去除率显著降低(P<0.05),应避免混合使用,而芽孢杆菌对戊二醛、阿维菌素不敏感,可以混合使用。

Different concentrations of 5 fishery drugs （potassium permanganate ,copper sulfate mixture , chlorine dioxide ,glutaraldehyde and abamectin） were added to the medium of bacteria ,the A600 and the nitrogen concentrations of liquid culture for 24 hours in order to study the influence of fishery drugs on the activity of denitrifying bacteria Pseudomonas stutzeri and Bacillus amyloliquef aciens .The results showed that denitrifying bacteria were most sensitive to potassium permanganate ,glutaraldehyde and chlorine dioxide ,significant decrease in nitrite removal rate ,biomass and the denitrification ability of denitrifying bacteria （P 〈 0 .05） at 4 mg/L of potassium permanganate ,0 .1 mg/L of glutaraldehyde ,and 0 .3 mg/L of chlorine dioxide （P 〈 0 .05） ,indicating that these drugs are not mixed in application .However ,the activity of the denitrifying bacteria was not affected by copper sulfate mixture and abamectin which can be mixed in application .B .amyloliquef aciens was most sensitive to potassium permanganate ,copper sulfate mixture ,and chlorine dioxide , significant decrease in removal rate of nitrite at 2 mg/L of potassium permanganate ,0 .25 mg/L of copper sulfate mixture ,and 1 mg/L of chlorine dioxide （ P 〈 0 .05） , indicating that these drugs are not mixed in the practical application .Glutaraldehyde and abamectin were not found to affect the activity of B .amyloliquef acien ,and be mixed for use .