青蒿B-BOX型锌指蛋白基因AaBBX22的克隆及表达分析

Cloning and Expression Analysis of AaBBX22 Encoding B-BOX-Type Zinc Finger Protein from Artemisia annua L

在外界环境刺激下B-BOX型锌指蛋白基因往往可以诱导植物相关基因的应答反应抵抗逆境胁迫。为了研究青蒿中AaBBX22基因在逆境胁迫中的表达特点,我们从青蒿的cDNA文库中克隆得到了AaBBX22基因。该基因全长为1 114bp,包含1个813bp的开放阅读框。生物信息学分析表明该基因具有2个典型的B-BOX型锌离子结合位点。进化树分析显示AaBBX22与葡萄和大豆的B-BOX型基因具有较近的亲缘关系。实时荧光定量PCR结果表明,在正常生长条件下,该基因在青蒿的各个组织部位中都有表达,其在根中的表达量最高,在花蕾中的表达量最低。盐和干旱处理都能显著诱导该基因的表达,脱落酸(ABA)、甲基茉莉酸(MJ)和低温处理显著抑制了该基因的表达,伤害处理在一定程度上抑制了该基因的表达。推测AaBBX22基因可能参与青蒿的耐盐和耐旱调控。

B-BOX-Type zinc finger protein usually induces expression of resistance to abiotic stress from the external environment. AaBBX22 was relative genes to respond to cloned from cDNA library of Artemisia annua L in order to study its expression and regulation under abiotic stress. AaBBX22 has a full length of 1 114 bp containing an ORF of 813 bp. Bioinformatics analysis reveals that AaBBX22 gene features two B-BOX motifs. Phylogenetic tree analysis indicates that AaBBX22 gene has high similarity to B-BOX-Type genes from Vitis vinifera and Glycine max. Real-time PCR analysis shows AaBBX22 expresses constitutively in all tissues of A. annua with the highest expression in roots and the lowest expression in buds. The expression of AaBBX22 could be induced by salt and drought treatments, however, ABA, MJ and cold treatments remarkably inhibits the expression of AaBBX22. Besides, wounding stress could inhibit the expression of AaBBX22 to some extent. It is speculated that the AaBBX22 mayparticipate in salt and drought tolerance regulation.