茶叶中有机硒的检测方法研究

Research of detection methods of organic selenium in tea

为确立茶叶中氢化物一原子荧光光谱法检测有机硒的含量，采用4＋1混合酸将茶叶中的有机硒氧化成无机硒后测定总硒含量；用1：1盐酸提取茶叶中的无机硒，并用4＋1混合酸消解，然后测定无机硒含量，再通过差减法获得有机硒的含量。结果表明，有机硒的检出限为4．89,．g／L，总硒加标回收率为86．74％～93．20oA，相对标准偏差（RSD）为0．83％～1．79％；无机硒加标回收率为81．00％～92．88％，相对标准偏差（RSD）为1．68％～2．23％。通过采用标准物质对照，偏差在合理的范围，该方法适合茶叶中有机硒和无机硒的测定。

Hydride generation atomic fluorescence spectrometry was used to detect the content of organic selenium in tea. Firstly, 4＋1 mixed acid was used to determine the total content of selenium by changing organic selenium to inorganic selenium in tea; then inorganic selenium was extracted with 1 ＂ 1 hydrochloric acid in tea, and was digested with 4-k 1 mixed acid to determine the content of inorganic selenium; finally the content of organic selenium was obtained by subtraction. The results showed that the detection limit of selenium is 4.89 /ug/L; the recovery rate of total selenium is 86.74%-93.20% by recovery experiment with detection