紫芝液体深层发酵条件的初步研究

Preliminary Study of Liquid Submerged Fermentation of Ganoderma sinensis

以紫芝为研究对象,讨论液体深层发酵碳源、氮源、无机盐和培养条件对菌体生物量的影响,确定紫芝液体发酵最优培养基配方为葡萄糖30,g/L、蛋白胨3,g/L、KH2PO41.2,g/L和MgSO4·7H2O 0.8,g/L.发酵条件为培养温度25,℃,起始pH自然,装液量24%,接种量15%,140,r/min培养7,d.同时,以菌体生物量、胞外多糖和pH为指标,绘制紫芝液态发酵动力学曲线,发酵培养7,d后菌体生物量和胞外粗多糖的含量分别达到最大值3.63,g/L和1.67,g/L.

The effect of nutritional and environmental conditions on mycelial biomass of Ganoderma sinensis in liquid fer- mentation were studied with a single factor and an orthogonal experiment. The results show that the best carbon source and nitrogen source are glucose and peptone respectively. Consequently, the optimal culture medium components were：30 g/L glucose, 3 g/L peptone, 1.2 g/L KH2PO4and 0.8 g/L MgSO4·7H2O. The optimum culture conditions were as follows：the liquid medium volume was 24%, inoculation quantity 15%, culture temperature 25 ℃, shaking frequency 140 r/min, and pH value was natural The liquid fermentation kinetics curve was determined by biomass, crude extrapolysaccharide and pH, and the optimization fermentation time was 7 d. Then the mycelium yield and extrapolysaccharide could reach 3.63 g/L and 1.67 g/L, respectively.