乌司他丁对百草枯中毒致兔肾损伤的保护作用

Protective effects of ulinastatin on kidney damage induced by paraquat poisoning in rabbit

目的:探讨乌司他丁对百草枯中毒致兔肾损伤的保护作用。方法:30只健康新西兰白兔随机分为单纯百草枯染毒组、乌司他丁干预组、0.9%氯化钠溶液对照组,每组10只。单纯百草枯染毒组及乌司他丁干预组以35 mg·kg-1剂量一次性腹腔注射百草枯溶液;对照组注射等体积0.9%氯化钠溶液;干预组注射百草枯1 h后于兔耳缘静脉缓慢注射乌司他丁100ku·kg-1,以后每天注射1次。染毒3 d后给予每只兔子行肾脏动态容积CT扫描,观察肾组织血流量(renal blood flow,RBF)变化、肾组织病理学改变及经耳缘静脉采血2 mL检测血清肌酐(Cr)。结果:与对照组和干预组相比,单纯百草枯染毒组RBF明显下降及Cr显著升高;与对照组相比,干预组RBF亦有下降、Cr亦有升高。光镜下单纯染毒组可见大量肾小球内皮细胞空泡变性、坏死等;干预组可见近端小管上皮细胞肿胀、空泡变性,肾小球内皮细胞无明显改变;对照组肾组织结构清晰。结论:乌司他丁通过改善肾脏循环以促进百草枯的排泄,产生对肾脏的保护作用。

Objective： To study the protective effects of ulinastatin on kidney damage induced by paraquat poisoning in rabbit. Methods： Thirty healthy New Zealand white rabbits were divided into paraquat poisoning group, ulinastatin group and 0.9% sodium chloride solution control group randomly, with 10 rabbits in each group. Paraquat poisoning group and ulinastatin group were given a single dose of paraquat solution 35 mg·kg^-1 by intraperitoneal injection, control group was given the same volume of 0.9% sodium chloride solution injected in peritoneal cavity. The ulinastatin group received the intravenous injection of ulinastatin 100 ku·kg^-1 after injection of paraquat for 1 hour, then the same dose of ulinastatin was given every day. Dynamic volume CT scan on kidney of each rabbit was given 3 days later for observing the changes of renal blood flow （RBF）. The renal tissue pathology was observed, and 2 mL blood was collected from ear marginal vein for detecting serum creatinine （Cr）. Results： Compared with the control group and intervention group, RBF decreased obviously and Cr increased significantly in paraquat poisoning group. RBF was higher and Cr was lower in intervention group compared with that of control group. Observed by light microscope, glomerular endothelial cell vacuolar degeneration and necrosis were found in paraquat poisoning group. While in intervention group, the proximal tubular epithelial cells were swelling and vacuolar degeneration, glomerular endothelial cells had no obvious change. Renal organizational structure was clear in control group. Conclusion ： Ulinastatin had a protective effect on rabbit kidney damage induced by paraquat poisoning, through improving renal circulation and promoting paraquat elimination.