LA检测隐球菌荚膜多糖抗原在隐球菌性脑膜炎诊断中的应用

Application of latex agglutination test for detecting cryptoceccal capsular polysaccharide antigen in diagnosis of patients with cryptoceccal meningitis

目的探讨乳胶凝集试验(LA)在隐球菌性脑膜炎中的早期诊断价值。方法收集56例确诊的隐球菌性脑膜炎患者和25例结核性脑膜炎、病毒性脑膜炎患者的脑脊液,采用乳胶凝集试验检测隐球菌荚膜多糖抗原,并与真菌培养和直接镜检方法相比较,评价其诊断的灵敏度和特异度。结果乳胶凝集试验、真菌培养和直接镜检3种方法诊断的灵敏度分别为91.1%、69.6%和73.2%,乳胶凝集试验特异度分别为96.0%、100%、100%。结论乳胶凝集试验检测隐球菌荚膜多糖抗原可作为隐球菌性脑膜炎的早期诊断方法。

Objective To explore the early diagnostic value of capsular polysaccharide antigens detected by latex agglutination test（LA） in the diagnosis of cryptococcal meningitis. Methods The cryptococcal capsular polysaccha antigens were tested by LA in the CSF from 56 patients with cryptococcal meningitis, and 25 patients with tuberculous meningitis or viral meningitis. The sensi- tivity and specificity were compared with fungal culture method and direct microscopy examination. Results The detection sensitiv- ity of latex agglutination, fungal culture and direct microscopy examination for diagnosis of cryptococcosis was 91.1%, 69.6% and 73.2%, respectively; and the specificity was 96.0%, 100% and 100%, respectively. Conclusion Detection of cryptococcal antigens by latex agglutination test could be considered as a test for early diagnosis of cryptococcal meningitis.