摘要
本文利用SRAP分子标记对国内8个鸭茅品种及野生材料进行快速鉴定和遗传多样性分析,结果表明,20对引物扩增出93个条带,多态性条带为72条,多态性条带比率为76.99％,每对引物组合的多态性带数平均为3.6条.GS值范围在0.7633 ～0.9232间,平均GS值为0.8547;聚类分析表明鸭茅的遗传多样性与地理分布密切相关,同时揭示出国产鸭茅品种遗传基础较为狭窄.此结果为国内鸭茅资源保护利用及新品种选育提供科学依据.
In this paper,fast identifications of domestic eight orchardgrass cultivars and wild orchardgrass were conducted,and their genetic diversity was analyzed by SRAP molecular markers.The result showed that 93 bands were produced by 20 primer combinations in SRAP,of which 72 bands （76.99 ％） were polymorphic,the average value of polymorphic bands per primer combination was 3.6.GS value range from 0.7633 to 0.9232,and the average value of GS was 0.8547.The result of cluster and principal component analysis indicated that the genetic diversity of orchardgrass were correlative with geographical distribution of accessions.In addition,the narrow genetic basic of Chinese orchardgrass cuhivars were found.The result would provide scientific basis for the resource protection and new cultivar breeding of Chinese orchardgrass.
出处
《西南农业学报》
CSCD
北大核心
2013年第5期2088-2092,共5页
Southwest China Journal of Agricultural Sciences
基金
国家"十二五"科技支撑计划项目(2011BAD36B01)"三峡库区优质肉牛安全生产关键技术集成与示范"
国家自然科学基金项目(31101760)"鸭茅抗秆锈病分子标记及核心种质构建"
关键词
鸭茅
分子标记
SRAP
遗传多样性
Orchardgrass
Genetic diversity
SRAP
Molecular markers
