摘要
为了解繁育基地养殖鲟鱼亲鱼的遗传背景,利用线粒体控制区部分序列对49个鲟鱼亲鱼中遗传多样性进行分析.结果表明:检鲟鱼亲鱼(目的序列长度412~420 bp)可以划分为3大类:E(达氏鲟,Acipenserdabryanus)、B(中华鲟,Acipenser sinensi)、D(史氏鲟,Acipenser schrencki)类,分别包括3、2、13个单倍型,多态位点分别有13、15、30个,单倍型多样度(Hd)分别为0.417、0.2、0.853,核苷酸多样性(Pi)分别为0.005 41、0.007 28、0.013 14,平均核苷酸差异数(K)分别为2.222、3、5.52.同类各单倍型之间的遗传距离为0.002 39 ~ 0.059 44,不同类鲟鱼单倍型之间的遗传距离在0.045 72 ~0.221 27.表明,3类鲟鱼亲鱼群体遗传多样性均较丰富.
Mitochondrial control region (D-loop) gene segments were applied to analyze and determine the genetic diversity of sturgeon broodstocks from a hatchery.The length of target sequences for the 49 individuals which were examined was 412-420 bp.All samples could be fallen into three groups:E(Acipenser dabryanus),B(Acipenser sinensis) and D(Acipenser schrencki) group,which consisted of 3,2 and 13 haplotypes,and existed 13,15 and 30 polymorphic sites,respectively.The haplotypic diversity(Hd) was 0.417,0.2 and 0.853,respectively.The nucleotide diversity(Pi) was 0.005 41,0.007 28 and 0.013 14,respectively.The average number of nucleotide differences(K) was 2.222,3 and 5.52,respectively.The genetic distance among intra-group and extra-group haplotypes changed from 0.00239to 0.05944,0.04572 to 0.22127,respectively.The results suggest that every group presents relatively high genetic diversity,and also propose that the parent fish should be selected from different haplotypes,aiming to reduce the inbred risk in fry production of sturgeon greatly.
出处
《西南农业学报》
CSCD
北大核心
2013年第5期2112-2115,共4页
Southwest China Journal of Agricultural Sciences
基金
贵州省科技计划课题"鱼类分子生物实验室建设"[黔科合农G字(2009)4011]
贵州省创新能力建设专项项目"贵州省渔业资源利用研发创新团队"[黔科合院所创能(2011)4004]
黔农科合创新基金"贵州山区鲟鱼养殖群体微卫标记的筛选及遗传结构分析"[2012015]
关键词
达氏鲟
中华鲟
史氏鲟
鲟鱼亲鱼
线粒体控制区
遗传多样性
Acipenser dabryanus
Acipenser sinensis
Acipenser schrencki
Sturgeon broodstocks
mtDNA D-Loop
Genetic diversity