胃黏膜组织肿瘤坏死因子-α、白细胞介素-1β在慢性胃炎脾胃湿热证患者中的表达及临床意义

Expression of Tumor Necrosis Factor-alpha and Interleukin-1 Beta in Gastric Mucosa of Chronic Gastritis Patients with Spleen-stomach Damp-heat Syndrome and Their Clinical Significance

【目的】探讨胃黏膜组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)在慢性胃炎脾胃湿热证中的表达及其临床意义。【方法】临床收集慢性胃炎患者72例,其中脾胃湿热证57例、脾气虚证15例,另招募志愿者12名作为正常对照组。胃镜下取胃窦黏膜,采用荧光定量PCR法检测各组TNF-αmRNA、IL-1βmRNA表达,酶联免疫吸附(ELISA)法检测TNF-α、IL-1β水平。【结果】脾胃湿热证患者TNF-α、IL-1β含量及其mRNA表达水平均较脾气虚证显著升高(P<0.05或P<0.01),呈一定程度的正相关。【结论】胃黏膜TNF-α、IL-1β的表达增强可能是慢性胃炎脾胃湿热证的病理基础之一。

Objective To observe the expression of tumor necrosis factor-alpha （TNF-α） and interleukin-1 beta （IL-1β） in gastric mucosa of chronic gastritis patients with spleen-stomach damp-heat syndrome and to explore their clinical significance. Methods Seventy-two chronic gastritis patients were enrolled into the study, which involved 57 patients with spleen-stomach damp-heat syndrome and 15 patients with spleen-qi deficiency syndrome. Additionally, 12 healthy volunteers served as the control. The levels of TNF-α and IL-1β in gastric mucosa were detected by enzyme-linked immunosorbent assay （ELISA）, and the expression of TNF-α and IL-1β mRNA was determined by real-time fluorescence quantitative polymerase chain reaction （PCR）. Results The levels of TNF-α and IL-1β and their mRNA expression in gastric mucosa of patients with spleen-stomach da rap-heat syndrome were higher than those in patients with spleen-qi deficiency syndrome （P〈0.05 or P〈0.01）, showing certain degrees of positive correlation. Conclusion The increased expression of TNF-α and IL-1β in gastric mucosa may be one aspects of the pathological basis of the occurrence of chronic gastritis patients with spleen-stomach damp-heat syndrome.