组成型表达S-腺苷甲硫氨酸合成酶基因提高转基因马铃薯抗旱耐盐碱性研究

Constitutive expression of S-Adenosyl-L-Methionine Synthetase gene enhanced tolerance to drought, saline and alkali in potato

以马铃薯品种大西洋无菌苗茎段为外植体,应用农杆菌介导法,组成型表达来源于野生大豆的S-腺苷甲硫氨酸合成酶(S-Adenosyl-L-Methionine Synthetase,GsSAMS)基因,获得PCR及RT-PCR阳性的转基因马铃薯。应用SAS和DPS数据处理软件对7个转基因株系与未转化株系分别经干旱、盐、碱胁迫及无胁迫后的株高、叶绿素含量、叶片相对保水率、相对电导率等形态及生理指标与产量进行统计分析。结果表明,SM22、SM4、SM33、SM13等4个马铃薯转基因株系具有较高的抗逆性,无论是逆境还是正常条件下栽培,产量均比未转基因植株有所增加。相关性分析结果显示,四种处理后马铃薯叶绿素含量、株高、相对电导率、相对保水率与产量具有线性相关性。

In this work, an S-AdenosyI-L-Methionine Synthetase gene isolated from wild soybean （GsSAMS） was constitutively expressed in potato cultivar Atlantic internodes via Agrobacterium-medi- ated transformation, and we got PCR and RT-PCR positive transgenic lines. Seven positive transformed and one untransformed potato lines were further selected, statistical analyses by SAS and DPS software were performed to identify relative plant length, chlorophyll content, leaf relative water content, relative conductivity and yield after drought, saline and alkali treatments were investigated. Among them, four transgenic lines SM22, SM4, SM33 and SM33 showed higher stress tolerance. Their yields were higher compared to the untransformed and the other three transformed lines under treatment, as well as under no treatment. Pearson correlation coefficients were generated between morphological and physiological indices and yield. Chlorophyll content, relative plant length, leaf relative water content and relative conductivity had linear relationship with yield.