用基于邻苯二甲醛柱前衍生的高效液相色谱法测定稻米的γ-氨基丁酸含量

Determination of γ- aminobutyric Acid Content in Rice by High Performance Liquid Chromatography Based on Pre- column Derivatization with o- phthalaldehyde

建立基于邻苯二甲醛（0-phthalaldehyde，OPA）柱前衍生的高效液相色谱法（highperformanceliquidchromato—graphy，HPLC），测定了稻米中Y-氨基丁酸（Y-aminobutyricacid，GABA）的含量。结果显示：用三氯乙酸作为提取溶剂的效果最好；检测限为0．5mg／L，提取回收率为79．1％-101．5％；巨胚糙米的GABA含量显著高于常规胚糙米的，发芽后巨胚糙米的GABA积累量也高于常规胚糙米的。

The high performance liquid chromatography （HPLC） method based on pre - column derivatization with o - phthal- aldehyde （OPA） was established, and it was used to determine the content of Y-aminobutyric acid （GABA） in rice. The results indicated that trichloroacetic acid used as solvent had the best extract effect; the detection limit of this method was 0.5 mg/L, and the recoveries were 79.1% - 101.5% ; the content of GABA in brown rice with giant embryo was significantly higher than that in brown rice with normal embryo; after germination, the accumulation of GABA content in brown rice with giant embryo was also high er than that in brown rice with normal embryo.