白细胞介素-10,-17及叉状/翼状螺旋转录因子,维甲酸相关孤儿受体-γt在子宫内膜癌中的表达及意义

Expression and Significance of Interleukin-10,-17,Forkhead/Winged Helix Transcription Factor and Retinoid- Related Orphan Receptors-γt in Endometrial Cancer

目的探讨白细胞介素(IL)-10,-17,叉状/翼状螺旋转录因子(Foxp3)及维甲酸相关孤儿受体(ROR)-γt在子宫内膜癌组织的表达及其与子宫内膜癌发生发展的关系。方法收集本院2012年12月至2013年2月的子宫内膜癌标本16例及良性子宫病变标本6例为研究对象,分别纳入子宫内膜癌组(n=16)与对照组(n=6)。采用链霉亲和素-生物素复合物免疫组织化学(SABC-IHC)染色法分别检测两组标本组织中IL-10,-17,Foxp3及ROR-γt的表达,采用IPP 6.0图像分析软件计算积分光密度(IOD)值,并结合临床病理特征对相关结果进行统计学分析(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该委员会批准,并与受试者签署临床研究知情同意书)。结果子宫内膜癌组IL-10,Foxp3的IOD值明显高于对照组,差异有统计学意义(P<0.05)。子宫内膜癌组中,ⅠB期、G2～G3级、有肌层浸润、有脉管癌栓者的IL-10,-17,Foxp3及ROR-γt的IOD值显著高于ⅠA期、G1级、无肌层浸润、无脉管癌栓者,差异有统计学意义(P<0.05);而在各组织类型间比较,则差异无统计学意义(P>0.05)。子宫内膜癌组与对照组IL-17,ROR-γt的IOD值比较,差异无统计学意义(P>0.05)。而子宫内膜癌组中,FIGO分期、组织分级、有无肌层浸润、有无脉管癌栓及病理类型方面比较,差异亦无统计学意义(P>0.05)。结论 IL-10,Foxp3的过表达参与子宫内膜癌的发生发展,IL-17,ROR-γt与子宫内膜癌发生发展无明显相关性。

Objective To explore the expression of interleukin（IL） -10, -17, forkhead/winged helix transcription factor （Foxp3） and retinoid-related orphan receptors （ROR）-Tt in endometrial carcinoma and their relationships with tumor progression. Methods From December 2012 to February 2013,a total of 16 samples of endometrial carcinoma tissues and 6 samples of benign uterine lesions tissues were selected,they were divided into endometrial carcinoma group （n = 16） and control group （n = 6）. Expression levels of IL-10, Foxp3, IL-17 and ROR-Tt detected by streptavidin-biotin complex immunohistochemistry （SABC-IHC）assay in two groups, integrated option density （IOD） value were calculated by IPP 6. 0 software, clinic-pathological characteristics were retrieved to make analysis, data analysis was completed by means of student test or variance analysis with SPSS 17.0 software. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of West China Second University Hospital, Sichuan University. Informed consent was obtained from all participants. Results Expression IOD value of IL-10, Foxp3 in endometrial carcinoma group were higher than those in control group, there had significance difference between two groups（P〈0.05）; In endometrial carcinoma group, IOD value of IL-10, 17,Foxp3 and ROR 7t of I B stage, Gg-G3, with myoinvasion, with vas tumor emboli were higher than those of I A stage, G1, without myoinvasion, and without vas tumor emboli, there had significance difference（P〈 0.05）. IOD value of IL 17 and ROR-Tt had no significance differences between two groups（P〈0.05）, nor among clinic-pathological characteristics（P〉0.05）. Conclusions High expression of IL-10 and Foxp3, not IL-17 and ROR-Tt, may participate in the genesis as well as the development of uterine endometrial cancer.