鱼腥蓝细菌PCC 7120 all 3555基因超表达和缺失突变体的构建及表型

Construction and phenotypic analysis of over-expression strain and deleted mutant strain of all 3555 in Anabaena PCC 7120

在鱼腥蓝细菌PCC 7120中,all3555是一个乙酰羟酸合成酶的编码基因,为研究该基因在鱼腥蓝细菌PCC 7120的生长和环境适应过程中的作用,构建了该基因的超表达和缺失突变菌株,结果显示该基因缺失后鱼腥蓝细菌PCC 7120仍可继续生长,但对NaCl胁迫的敏感性有一定程度提高,而超表达该基因则会提高鱼腥蓝细菌PCC 7120对氧化胁迫和NaCl胁迫的抗性,表明all3555对鱼腥蓝细菌PCC 7120的正常生长影响较小,但对菌株的抗氧化胁迫和抗NaCl胁迫等有重要作用。

To study the function of the al13555, an over-expression strain and a deleted mutant strain of a113555 in Anabaena PCC 7120 was constructed. The defected mutant strain was found to grow in usual condition but be more sensitive to NaC1 under salt-stress. When all3555 was over-expressed, the resistance of Anabaena PCC 7120 to oxidative stress and NaC1 under salt-stress was improved. Results showed that all3555 almost didn＇t affect the growth of Anabaena PCC 7120 under the normal condition, but it was important for the resistance of Anabaena PCC 7120 to oxidative stress and NaC1 under the salt-stress.