冬凌草叶和茎中冬凌草甲素含量的对比研究

Determination of Oridonin in Leaves and Stems of Blushred Rabdosia by HPLC

目的 :建立了冬凌草叶及茎中冬凌草甲素的高效液相色谱测定法。方法 :采用HypersilODS 2C18色谱柱 ,以甲醇 -水 (5 0∶5 0 )为流动相 ,在 2 38nm波长处检测。结果 :叶和茎中冬凌草甲素的含量分别约为 0 5 5 %和0 0 2 5 % ,平均回收率为 97 45 %。结论 :该方法准确、灵敏、简便。

Objective:HPLC method was established for the determination of oridonin in leaves and stems of Blushred Rabdosia.Method:The chromatographic system consisted of Hypersil ODS 2 C 18 Column and mobile phase of MeOH-H 2O(50∶50)with a detection wavelength at 238 nm.Results:The contents of oridonin in leaves and stems were found to be about 0 55% and 0 025%,respectively.The average recovery was 97 45%.Conclusion:This method was simple,sensitive,accurate and precise.