基因测序在人类腺病毒分型中的应用

Application of gene sequencing in typing of human adenovirus

人类腺病毒可引起呼吸道、胃肠道和眼部的感染,不同类型的腺病毒引起的疾病类型及表现不尽相同.因此,腺病毒的分型具有重要的临床研究价值.在基因学上,编码六邻体蛋白,五邻体蛋白和纤维蛋白的区域是腺病毒基因组中变异最大的区域,通过对其编码区的测序,尤其是六邻体蛋白编码区的测序,是快速可靠的确定腺病毒类型的重要方法之一.此文主要对基因测序在腺病毒分型中的优势、应用现状,在腺病毒突变重组、种系发生学中的鉴定检测等作一综述.

Human adenovirus can cause respiratory,gastrointestinal and eye infections.Different adenovirus types can result in different diseases and clinical manifestations.Therefore,adenovirus classification is important for clinical research.Coding region of hexon,penton and fibrin is the largest region of genome variation in adenovirus,and coding region sequencing is one of the rapid and reliable methods for gene typing,especially for hexon protein.The advantage and application of gene sequencing in adenovirus typing,the mutants or recombinants testing and species identification are summarized in this article.