解毒通络方抗大鼠脑缺血再灌后脂质过氧化损伤的实验研究

Experimental Study of the Effects of Jiedutongluo Decoction on the Lesions Caused by the Lipid Peroxidation after Cerebral Ischemia Reperfusion in Rats

为了探讨解毒通络方防治大鼠脑缺血再灌后DND的机理 ,采用大鼠双侧颈总动脉结扎合用腹腔注射硝普钠为模型 ,实验分为正常、模型、解毒通络方、尼莫地平 4组 ,用光化学方法检测造模后脑组织超氧化物歧化酶 (SOD)活性、丙二醛 (MDA)含量。发现模型组较正常组大鼠脑组织SOD降低 ,MDA升高 ,用解毒通络方、尼莫地平后可以明显提高SOD活性 ,降低MDA含量 ,前者较后者效果更明显。说明解毒通络方与尼莫地平均具有抗脂质过氧化损伤的能力 。

This experiment is designed to study the preventive and treating effects of Jiedutongluo Decoction (JD) on delayed neuronal death caused by cerebral ischemia reperfusion in the rats which underwent bilateral common carotid blockage and intraperitoneal injection of sodium nitroprusside. The rats in the experiment were divided into control group, model group, JD treating group and nimodipine treating group. The cerebral SOD activity and MDA level were determined by photochemical method. The results showed that compared to that in the rats in the control group, the SOD activity was lower and the MDA level was higher in the rats in the model group; and both JD and nimodipine could increase the SOD activity and decrease the MDA level, but the effects of JD was stronger than those of nimodipine, which implies that the two drugs have anti-lipid-peroxidating effects, and JD is more effective than nimodipine.