复合应激因素致大鼠疲劳模型海马氨基酸含量的变化及中药的调节作用

The Rat Fatigue Model Induced by Combined Stress Factors - Changes of the Levels of Amino Acids in the Hippocamp and the Regulating Effects of Traditional Chinese Drugs on Them

观测了复合应激因素致慢性疲劳动物模型海马组织中 16种游离氨基酸含量的变化及中药的调节作用。结果显示 :①模型动物的甘氨酸、丙氨酸、赖氨酸、精氨酸、牛磺酸及γ -氨基丁酸的水平显著升高 ,但服用消疲怡神口服液及人参皂甙片后 ,升高的氨基酸无明显下调 ,说明单纯用上述氨基酸浓度的变化还不能完全解释该模型的行为改变及中药的作用机制。②模型大鼠海马中的Glu/GABA及兴 /抑比值明显下降 ,从氨基酸递质来看 ,此时的中枢以抑制占优势 ,与动物的行为表现一致 ;服药后 ,Glu/GABA比值明显上调 ,兴 /抑比值也出现上调趋势 ,尤以人参皂甙的作用明显 ,说明消疲怡神口服液与人参皂甙均能通过调整兴奋性与抑制性氨基酸的水平及比值 ,对抗因慢性复合应激因素所致疲劳、记忆力下降及抑郁倾向的情绪改变。

An observation on the changes of the levels of 16 amino acids in the hippocamp of the rat with chronic fatigue induced by combined stress factors, and the regulating effects of traditional Chinese drugs on the 16 amino acids, was made in the experiment. The results showed that both Yishen Oral Liquid and Ginsenoside Tablets could not significantly decrease the increased levels of glycine, alanine, lysine, arginine, taurine and γ-aminobutyric acid in the rat, but the two drugs, especially Ginsenoside Tablets, could significantly increase the decreased ratios of Glu/GABA and excitation/inhibition.