Sorcin蛋白高表达与肺癌细胞株NCI-H446多药耐药的关系

Relationship of Sorcin over- expression to multidrug resistance of human lung cancer cell line NCI- H446

目的探讨Sorcin高表达与肺癌细胞NCI-H446多药耐药的相关性。方法研究对象为肺癌细胞NCI-H446,以顺铂为诱导药物,建立多药耐药细胞系NCI-H446/CDDP;应用反义核酸技术抑制NCI-H446/CDDP细胞Sorcin的表达建立转染细胞,为反义核酸组,同时设空白对照组。通过RT-PCR及Western-blot分别检测各组细胞中Sorcin在核酸及蛋白水平的表达;MTT法测定NCI-H446组、反义核酸组、NCI-H446/CDDP组3组细胞在不同化疗药物DDP、VCR、ADM、VP-16作用下生存率;用Pearson相关分析Sorcin高表达与肺癌细胞株NCI-H446多药耐药的相关性。结果各组细胞中Sorcin在核酸及蛋白水平的表达,NCI-H446/CDDP组与空白对照组比较,差异无显著性意义(P>0.05),NCI-H446组、反义核酸组、NCI-H446/CDDP组各组间比较均具有显著性意义(P<0.05);NCI-H446/CDDP细胞中Sorcin在核酸及蛋白水平均明显高于NCI-H446细胞,分别为NCI-H446细胞的9.81倍和7.67倍。各组细胞在不同化疗药物作用下IC50:NCI-H446/CDDP组>反义核酸组>NCI-H446组,各组间比较差异具有显著性意义(P<0.05)。Pearson相关提示:Sorcin核酸及蛋白水平的表达与在不同化疗药物作用下各组细胞的IC50值呈负相关(P<0.05)。结论 Sorcin蛋白的高表达与肺癌多药耐药相关,抑制Sorcin蛋白的表达可以逆转肺癌多药耐药性。

Obsjective To investigate the relationship of the high expression of sorcin with muhidrug resistance in lung cancer cell NCI - H446. Methods Lung cancer cell NCI - H446 was object of study, set up cell line NCI - H446/CDDP through cisplatin;Using antisense nucleic acid technology inhibited the expression of sorcin in NCI - H446/CDDP cell, building antisense nucleic acid group, at same time setting up blank control group. RT - PCR and Western - blot were used to analyze the expression of sorcin at mRNA and protein levels in every cell group. MTT was used to three groups cell （NCI - H446 cell group, antisense nucleic acid group, NCI - H446/CDDP group） survival rate in DDP, VCR, ADM, VP - 16. Pearson correlation was used to analyze the correlation of the high expression of sorcin with muhidrug resistance in lung cancer cell NCI - H446. Results Comparison with the expression mRNA and protein of sorcin in every groups, the re- sult showed ： comparing NCI - H446/CDDP group with blank control group has no statistical significance （ P 〉 0.05）, Intercomparison in NCI - H446 cell group, antisense nucleic acid group, NCI - H446/CDDP group has statistical significance （ P 〈 0.05 ）. The expression mRNA and protein of sorein in NCI - H446/CDDP were obviously higher than it in NCI - I-I446 cell, 9.81 and 7.67 times respectively. The result of IC50： NCI - H446/CDDP group 〉 antisense nucleic acid group 〉 NCI- H446 cell group, has statistical significance （P 〈 0.05 ）. Pearson correlation to point out： the expression of sorcin in mRNA and protein was negatively correlated to IC50 of the group cells in different chemotherapy drugs. Conclu- sion The high expression of sorcin is related to muhidrug resistance in lung cancer cell, Inhibition of its expression can reverse the drug resistance of lung cancer.