高压氧治疗对脊髓损伤大鼠脾脏组织病理的影响

Effects of hyperbaric oxygen therapy on splenocytes in rats with spinal cord injury

目的：探讨高压氧治疗对脊髓损伤大鼠脾脏组织病理的影响。方法：40只SD大鼠随机数字表法分为4组：空白对照组（n=10）、脊髓损伤对照组（n=10）、高压氧处理对照组（n=10）和脊髓损伤高压氧治疗组（n=10）。脊髓损伤对照组和脊髓损伤高压氧治疗组采用改良Allen’s法制作T8～T9急性脊髓损伤大鼠模型。空白对照组和脊髓损伤对照组大鼠正常饲养。不进行高压氧处理；高压氧处理对照组和脊髓损伤高压氧治疗组大鼠每间隔24h进行1次高压氧处理，每天1次，连续14d。实验结束后，取各组大鼠脾脏标本电子显微镜下进行观察。结果：空白对照组大鼠脾细胞排列整齐、规则，结构清晰，细胞核居中，呈椭圆形。脊髓损伤对照组较空白对照组大鼠脾脏淋巴细胞、网状细胞等实质细胞弥漫性减少，胞质密度不均匀。血管内红细胞淤积，脾细胞凋亡后被胶质细胞等替代。高压氧处理对照组大鼠脾细胞间隙增宽，胞浆、胞质均匀，细胞排列相对整齐、规则，结构清晰，细胞核居中，呈椭圆形。脊髓损伤高压氧治疗组大鼠脾细胞及淋巴细胞增殖旺盛．细胞核染色深，细胞排列致密且较规则，细胞间隙小。结论：高压氧治疗对大鼠脊髓损伤后脾细胞损伤具有保护作用。

Objective：To explore the effects of hyperbaric oxygen therapy on splenocytes in rats with spinal cord injury. Methods： Forty SD rats were randomly allocated to the normal control group（ group NC, n = 10）, spinal cord injury control group（ group SCI ,n = 10）, hyperbaric oxygen treatment control group （group HOT, tt = 10） and hyperbaric oxygen therapy for spinal cord injury group （group SCI-HOT, n = 10）, respectively. In groups SCI and SCI-HOT, the modified Allen＇s method was employed to construct the acute Ts-T9 spinal cord injury model in rats ,whilst hyperbaric oxygen therapy was spared in groups NC and HOT. In groups HOT and SCI-HOT, hyperbaric oxygen therapy was administered once daily every other 24 h for 14 consecutive days. The spleens of all groups were subjected to electron microscopic examination at the end of the study. Results： In group NC, the splenocytes appeared well-ordered and had a characteristic architecture, with their oval-shaped nuclei being positioned centrally to the cell. Apart from a ubiquitously reduced number of parenchymal cells（ i. e. lymphocytes and reticular cells） whose cytoplasmic density was heterogeneous, group SCI was characterized by intravascular red blood cell stagnation and a preponderance of gliocytes following splenocyte apoptosis. In group HOT, the splenocytes were noted as having a widened intercellular gap and homogeneous cytoplasm. These splenocytes were well-ordered and had a characteristic architecture, with their oval-shaped nuclei being positioned centrally. Intensive proliferation of splenocytes and lymphocytes which were densely and regularly ordered, with heavily stained nuclei and reduced intercellular gap, was demonstrated in group SCI-HOT. Conclusion： Hyperbaric oxygen therapy has a protective effect on spienocyte injury in rats with SCI.