谷氨酰胺对应激大鼠学习记忆的影响及其机制的研究

EFFECT OF GLUTAMINE ON LEARNING AND MEMORY OF STRESSED RATS AND THE MECHANISM INVOLVED

目的 观察谷氨酰胺 (Gln)对应激大鼠学习记忆的影响 ,并对其机制进行探讨。方法 Wistar大鼠 30只 ,随机分为三组 : 组为对照组 , 组为应激组 , 组为应激 +Gln组。其中 、 组饲以普通饲料 , 组给予补充 2 % Gln的饲料。以光电刺激作为应激条件 ,在 组和 组大鼠建立应激模型 ,用 Y-迷宫法对大鼠的学习记忆能力进行测定 ,并分别用放射性配基结合法、放射免疫法、荧光分光光度计法测定大鼠小脑中 γ-氨基丁酸 (GABA)受体、脑中胆囊收缩素 (CCK)、还原型谷胱甘肽 (GSH)的含量。结果 与 组相比 , 组大鼠在 Y-迷宫实验中的逃避时间延长 ,而 组与 组则无显著差异 ; 组大鼠小脑中 GABA受体水平、CCK含量均升高 ,而 GSH含量则降低 ,给予 Gln后可从一定程度上逆转上述改变。结论 心理应激可影响大鼠的学习记忆能力 ,而 Gln则有一定的改善作用 ,其机制可能是通过减轻应激所致的脑中GABA受体水平、CCK和

Objective:To investigate the effects of glutamine(Gln) on learning and memory of the stressed rats and the mechanism involved. [WT5FZ]Methods: [WT5BZ]Three groups of rats were fed on normal diet(group Ⅰ、Ⅱ) or 2% Gln supplemented diet(group Ⅲ) 2 weeks later, the stress model was established in group Ⅱ and Ⅲby infliction with irregular light and electric shock. The learning and memory of rats were observed using Y maze test. The level of γ aminobutryic acid(GABA) receptors in cerebellum and contents of cholecystokinin(CCK), glutathione(GSH) in rat brain were assayed. [WT5FZ]Results: [WT5BZ]The avoidance time of the stressed rats was increased in Y maze test. The B max of GABA receptor in cerebellum and level of CCK in brain were significantly increased in the stressed rats whereas brain GSH content was decreased. Administration with Gln could alleviate or reverse those changes. Conclusion: Gln can improve learning and memory of rats under stress conditions through mitigating the disorder of brain metabolism caused by stress.