喉癌中p16和CDK4表达的研究

EXPRESSION OF p16 AND CDK4 IN LARYNGEAL CARCINOMA

目的 研究喉癌中 p16、CDK4蛋白的表达情况及其与临床病理特点的关系。方法 以抗 p16、抗 CDK4抗体为标记物 ,采用 SP(Streptavidin Peroxidase)免疫组织化学方法对 2 8例喉癌及 13例声带息肉石蜡标本进行分析。结果 p16在喉癌和声带息肉中的阳性率分别为 6 0 .71%和10 0 % (P<0 .0 1) ;CDK4在喉癌和声带息肉中的阳性率分别为 6 4.2 9%和 2 3.0 8% (P<0 .0 5 ) ;p16在喉癌 级组和 级以上组中的阳性率分别为 87.5 %和 2 5 % (P<0 .0 1)。CDK4在喉癌 级组和 级以上组中的阳性率分别为 5 6 .2 5%和 75 % (P>0 .0 5 )。结论 p16及 CDK4蛋白在喉癌的发生中可能起重要作用 。

Objective To investigate the expression of p16 and CDK4 proteins and their relationship with the clinical pathology of laryngeal carcinoma. Methods The expression of p16 and CDK4 were studied in 28 cases of laryngeal carcinoma and 13 cases of vocal cord polyps using SP (streptavidin peroxidase) immunohistochemical method. Results The percentage of p16 positive staining in laryngeal carcinoma and vocal cord polyps were 60.71% and 100% respectively ( P <0 01), while those of CDK4 were 64.29% and 23.08%( P <0 05). Percentage of p16 positive stanining in laryngeal carcinoma of grade Ⅰ and those of higher grades were 87.5% and 25% respectively ( P <0 01), while those of CDK4 were 56.25% and 75%( P >0 05).Conclusion Both p16 and CDK4 may be play an important role in laryngeal carcinogenesis. Expression of p16 is correlated with the clincial pathology of laryngeal carcinoma.