参麦合剂对兔干眼症模型泪液分泌及眼表结构的影响

Effect of Shenmai-mixture on Tear Secretion and the Ocular Surface Structure on Rabbit Dry eye Model

目的:探索参麦合剂对兔干眼症模型泪液分泌及眼表结构的影响。方法:将30只健康新西兰大白兔随机分为空白对照组、实验对照组、药物治疗组。制作干眼动物模型后,各组给予相应治疗。治疗前和治疗后第7、14、21、28 d分别测定Schirmer试验值,BUT值、眼表荧光素染色(FL)、结膜印记细胞学检查(CIC),观察眼表泪膜状态和细胞学变化。治疗28 d后,每组随机选取5只动物,处死后剪下角结膜组织,行角结膜石蜡切片光镜观察。结果:药物治疗组用药14 d后,Schirmer值及BUT值均较其他两组明显增加(P<0.01);结膜细胞印迹学检查发现药物治疗组结膜杯状细胞数量较其他两组明显增多;光镜检查发现药物治疗组与其他两组相比,部分结膜上皮层数增多、杯状细胞增多。结论:参麦合剂能明显增加兔干眼症模型泪液分泌、改善泪膜稳定性和眼表细胞结构。

Objective： To explore the effects of Shenmai-mixture on tear secretion and the ocular surface structure of rabbit dry eye model.Methods： Thirty healthy New Zealand white rabbits were divided randomly into 3 groups： control group,experimental control group and drug-treated group.After the rabbit dry eye model was established,the control group were not interfered,the experimental control group received saline intragastric administration,the drug treatment group received Shenmai mixture intragastric administration.On day 0,7,14,21 and 28,Schirmer test,tear break-up time（BUT）,fluorescein staining of the ocular surface were measured and conjunctival impression cytology specimens were collected.All of these were used to observe the state of the tear film and ocular surface changes.Subjects underwent conjunctival imprint cytology inspection to observe the ocular surface change in cytology.On day 28,cornea and conjunctiva were harvested for Hematoxylin and Eosin（HE） staining,scanning observation.Results： The Schirmer test value and BUT value in drug-treated group were significantly longer than those in other two groups on day 14（P 0.01）.After intragastric administration of Shenmai-mixture for 28 days,c onjunctival imprint cytology showed the conjunctival goblet cells in the drug-treated group increased significantly than the other two groups.Epithelial layers and conjunctival goblet cells increased as shown by the HE staining.Conclusion： Shenmai-mixture can be used to treat dry eyes because it increase tear secration,improves stability of tear and change the structure of ocular surface.