几种药食兼用植物提取物抗氧化作用的体外研究

Experimental Studies on Antioxidation of Extracts from Several Plants Used as Both Medicines and Foods in vitro

目的:对几种药食兼用植物红茶、绿茶、乌龙茶、丁香、葡萄皮/籽、姜黄的提取物以及茶多酚和儿茶素制品进行体外抗氧化作用的测定。方法:采用化学发光法、超微弱发光技术和巨噬细胞呼吸爆发实验。结果:提取物对超氧阴离子(O_2)的清除能力为:丁香>红茶>绿茶>葡萄皮/籽>乌龙茶>姜黄;对羟自由基(·OH)的清除能力为:红茶>丁香>绿茶>葡萄皮/籽>乌龙茶>姜黄;除丁香外,其余提取物对·OH的清除能力均大于对O_2的清除。茶多酚和儿茶素制品对O_2和·OH亦具有很强的直接清除能力。经对活性较强的丁香、红茶和儿茶素进一步研究,发现红茶对大鼠腹腔巨噬细胞呼吸爆发的抑制作用大于丁香。超微弱发光测定结果显示,被试的丁香、红茶、茶多酚和儿茶素均可延迟和抑制CuSO_4-Phen-Vc-H_2O_2-DNA体系导致的DNA损伤。结论:被试的提取物及制品均有清除氧自由基的作用,其中丁香、红茶和儿茶素活性较强,并具有阻断DNA氧化损伤链反应和预防性的效果。

Objective: The scavenging effects of 8 extracts from several plants used as both medicines and foods on oxygen free radicals were studied in vitro . Methods: Chemiluminescence (CL) method and ultra-weak luminescence technique were used, meanwhile, macrophage respiratiory burst was also carried out to evaluate the antioxidation reaction. Results: The scavenging activity on superoxide anions (O2) differed in the following order: extract of clove (CE) > black tea (BTE) > green tea (GTE) > grape peel/seed ( GE)≥oologn tea ( OTE) > > turmeric (TE) . In Fenton reaction for hydroxyl radicals ( OH) , the order as follows: BTE> CE≥GTE> GE> OTE> > TE. Most extracts have stronger eliminating efficiency on ' OH than on O2 except CE, which may scavenge the O2 formation more efficiently. After macrophages (5×106 cells/ml) were stimulated with phorbol myris-tate acetate, BTE showed stronger inhibiting action than CE on respiratory burst in macrophages. The results of ultra-weak luminescence measurement indicated that the extracts could delay and depress the DNA damage in CuSO4-Phen-Vc-H2O2-DNA chemi-luminescence system. Conclusions: It suggests that the tested antioxidants could decrase the formation of O2 or ' OH, some have chain-breaking function and preventative function during DNA damgage.