黄参方剂诱导胃癌癌前病变细胞凋亡和影响BCL-2蛋白表达的研究

Research on the effect of huang shen preparation on the induction of apoptosis of gastric pre-carcinoma and the expression of BCL-2 protein

目的 :探讨黄参方剂对胃癌癌前病变细胞凋亡及相关基因表达的影响。方法 :将 72例胃癌癌前病变患者随机分为 2组 ,治疗组 37例 ,口服黄参方剂 6 0ml/d ;对照组 35例 ,口服维酶素片 12片 /天 ,疗程 2月。治疗后胃镜同一部位取材 ,检测治疗前后细胞凋亡指数和BCL 2蛋白的表达。采用原位末端标记法 (TUNEL)检测细胞凋亡 ,采用免疫组化检测BCL 2蛋白的表达。结果 :黄参方剂组服药前后细胞凋亡指数分别为 (19 8± 9 6 ) %和 (32 8± 16 2 ) % ,服药后细胞凋亡指数高于服药前 (P <0 0 5 )。BCL 2蛋白阳性率治疗前后分别为 5 2 8%和 2 6 4% ,治疗前后比较具有显著性差异 (P <0 0 5 )。而对照组细胞凋亡指数和BCL 2蛋白阳性率在服药前后比较均无显著性差异 (P >0 0 5 )。结论 :黄参方剂可诱导胃癌癌前病变细胞凋亡。

Objective:To evaluate the effects of Huang Shen prearation on apoptosis and expression of Bcl-2 in gastric precancerous lesions.Methods:72 cases of gastric precancerous lesions were divided into two groups at randoma,treatment group of 37 cases and a control group of 35 cases.The treatment group was treated with Huang Shen preparation 60ml a day for two months.The control group was treated with wei mei su 12 tablets a day for two months.TUNEL method was used to detect apoptosis.Immunohistochmical analysis was used to detect the expression of BCL-2 protein.Results:The treatment group changed as follow:Apoptosis index increased from (19.8±9.6)% to (32.8±16.2)%,the expression rate of BCL-2 decreased from 52.8% to 26.4%,The change of apoptosis indexes and expression rate of BCL-2 were statistically significant (P<0.05).In control group it did not change significantly (P<0.05).Conclusions:It is necessary to study the therapeutic value of Huang Shen preparation on gastric precancerous lesions,since it can induce apoptosis of gastric precancerous lesions cells.