聚合酶链反应对颈部肿块的EBV检测

Detection of Epstein Barr virus DNA in fine needle aspiration specimen from cervical lymphnodes with polymerase chain reaction

目的 :探讨聚合酶链反应 (PCR)对隐匿性鼻咽癌的诊断意义。方法 :采用 PCR检测 58例颈部肿块的细针抽吸标本中的 EB病毒 (EBV)。结果 :35例颈中、上淋巴结转移癌 2 8例 EBV阳性 ,3例淋巴瘤阴性 ,4例锁骨上淋巴结转移癌阴性 ;1 6例淋巴结炎性病变 1例为弱阳性 ,1 5例均为阴性 ;该法诊断鼻咽癌的灵敏度为 89.3% ,特异性为 86.7%。结论 :用 PCR检测颈部转移癌中的EBV- DNA,对隐匿性鼻咽癌的诊断具有一定的临床意义。

Objective:For the diagnosis of occult nasophargngeal carcinoma. Method:Epstein Barr virus in 58 fine needle aspirations from cervical metastatic lymphnodes were detected with polymerase chain reaction. Result:The positive reaction was found in 28/35 metastatic carcinoma samples from middle or upside neck.Negative reaction was found in 3 lymphomas,4 metastatic carcinomas in clavicular superior lymphnodes and 15/16 inflammatory diseases of cervical lymphnodes. The sensibility of this method for the diagnosis of nasopharyngeal carcinoma was 89.3 %. The specificity was 86.7 %.Conclusion: The detection of Epstain Barr virus in metastatic carcinoma from cervical lymphnodes had some clinical values for the diagnosis of occult nasopharyngeal carcinoma.