兔肝脏、肌肉、皮下VX2肿瘤模型的建立和对比研究

The establishment of rabbit models with intrahepatic,intra-muscular and subcutaneous VX_2 tumor:a comparison study

目的通过对肝脏、肌肉及皮下不同部位兔VX2肿瘤模型的建立和比较,寻求更适合于肿瘤经皮介入治疗的荷瘤动物模型。方法将54只新西兰白兔按VX2肿瘤接种部位不同随机分为肝脏组、肌肉组和皮下组,每组18只。三组动物术后12、16、20 d均进行CT灌注扫描,测量肿瘤大小,每次各组分别处死6只兔进行病理观察,并对三组模型的建模手术时间、肿瘤体积及并发症等情况进行比较。结果皮下组肿瘤生长最慢,肌肉组其次,肝脏组生长最快,组间差异有统计学意义(P<0.05)。接种第12天,肝脏组肿块长径已约1 cm,而皮下组则需20 d肿块长径才达1 cm左右,肌肉组肿块生长速度介于二者之间。三组建模所用时间分别为肝脏组(5.54±0.51)min,肌肉组(1.02±0.20)min,皮下组(0.98±0.21)min,肝脏组明显较长。除了肝脏组出现腹水、肝内转移及腹壁种植外,皮下组、肌肉组均无明显影响模型的情况。结论与肝脏和皮下建模相比,兔肌肉内VX2肿瘤模型具有操作简单,成瘤稳定,肿瘤生长速度适中的优点,更适合作为兔VX2瘤的经皮介入治疗模型。

Objective To find out the tumor- bearing rabbit model which is most suitable for percutaneous interventional therapy by comparing the models receiving intrahepatic, intra- muscular or subcutaneous inoculation of VX-2 tumor. Methods A total of 54 New Zealand rabbits were randomly and equally divided into three groups. Rabbits of group A （n = 18） received intra-hepatic inoculation of VX-2 tumor. Rabbits of group B （n = 18） received intra-muscular inoculation of VX-2 tumor and rabbits of group C （n = 18） were subcutaneously inoculated with VX- 2 tumor. Multi- planar CT perfusion- scanning was performed on the 12~, 16~ and 20~ day after inoculation to determine the tumor size. Every 6 rabbits from each group were sacrificed each time at the 12^th, 16^th and 20^th day after inoculation. The specimens were collected and sent for pathologic examination. The operation time for complete modeling, the tumor size and the modeling-related complications were compared among the three groups. Results The VX- 2 tumor of group A grew most fast and VX-2 tumor of group C grew slowest. The difference among the three groups was statistically significant （P 〈 0.05）. On the 12^th day after inoculation the tumor size of group A was already about one cm in diameter, while in group C and group B the tumor size became one cm in diameter on the 20d, day and 16^th day after inoculation respectively. The operation time needed for modeling for group A, B and C was （5.54 ± 0.51） rain, （1.02 ± 0.20） rain and （0.98 ± 0.21） rain respectively. The operation time needed for modeling of group A was the longest. Aseites, intrahepatie metastases as well as tumor implantation of abdominal wall were found in group A, while no obvious untoward events affecting VX-2 tumor model were observed in both group B and C. Conelusion Compared to intrahepatic and subcutaneous inoculation VX- 2 tumor model, the establishment of intra-muscular inoculation VX-2 tumor model of rabbit is more simple in manipulation with higher tumor-formation rate, besides the growth rate of the tumor is moderate. Therefore, intra- muscular inoculation VX- 2 tumor model of rabbit is most suitable for percutaneous interventional study.