TSLC1、DAL-1/4.1B和MPP3在大肠癌中的表达及其临床意义

Clinical significance of expression of TSLC1, DAL-1/4.1B and MPP3 in colorectal carcinoma

目的:研究肺癌肿瘤抑制物1(tumor suppressor i n lung cancer-1,TSLC1)、肺腺癌差异表达因子4.1B(differentially expressed in adenocarcinoma of the lung/4.1B,DAL-1/4.1B)和果蝇肿瘤抑制因子同源分子-3(membraneprotein palmitoylated 3,MPP3)在大肠癌中的表达及其相互关系,并探讨其临床病理意义.方法:采用免疫组织化学EnVisionTM法检测76例大肠癌和22例正常肠黏膜组织中TSLC1、DAL-1/4.1B、MPP3的表达,并结合其临床病理特征分析.结果:正常肠黏膜组织中TSLC1、DAL-1/4.1B和MPP3均呈清晰的棕黄色染色定位在上皮细胞质和/或细胞膜.TSLC1、DAL-1/4.1B和MPP3蛋白在大肠癌中的阳性表达率均明显低于其在正常肠黏膜组织中的表达(32.89%vs81.81%,27.63%vs 63.64%,35.53%vs 68.18%;P<0.05).TSLC1、DAL-1/4.1B和MPP3蛋白在大肠癌的表达缺失与肿瘤的分化程度、浸润深度、淋巴结转移和Dukes'分期密切相关(P<0.05),而与患者的性别、年龄和肿瘤大小无关.结论:大肠癌中存在TSLC1、DAL-1/4.1B和MPP3基因的表达缺失,TSLC1与DAL-1/4.1B及MPP3之间的相互作用可能是其发挥肿瘤抑制作用的主要分子机制,三者可能通过TSLC1级联反应共同参与大肠癌的发生、发展和转移.

AIM： To investigate the clinicopathological significance of expression of tumor suppressor in lung cancer-1 （TSLC1）, differentially expressed in adenocarcinoma of the lung/4.1B （DAL-1/4.1B） and membrane protein palmitoylated 3 （MPP3） proteins in colorectal carcinoma. METHODS： Immunohistochemistry was used to detect the expression of TSLC1, DAL-1/4.1B and MPP3 in 76 colorectal carcinoma tissue samples and 22 normal colorectal mucosal tissue samples. The correlation between expression of TSLC1, DAL-1/4.1B and MPP3 and clinicopathologic features of colorectal carcinoma was analyzed. RESULTS： Positive staining for TSLC1, DAL-1/4.1B and MPP3 was observed in the cytoplasm and/or membrane of epithelial cells in normal colorectal mucosal tissue. The positive rates of TSLC1, DAL-1/4.1B and MPP3 expression were significantly lower in colorectal carcinoma than in normal control tissue （32.89% vs 81.81%, 27.63% vs 63.64%, 26.32% vs 68.18%; all P 〈 0.05）. The lower expression of TSLC1, DAL-1/4.1B and MPP3 in colorectal carcinoma showed a significant correlation with degree of differentiation, depth of infiltration, lymph node metastasis and Dukes＇ stage （all P 〈 0.05）, but not with gender, age or tumor size. CONCLUSION： Low expression of TSLC1, DAL-1/4.1B and MPP3 may be involved the in the development and metastasis of colorectal carcinoma.